Production and characterization of a bacteriocin-like inhibitory substance produced by indigenous soil associated Pseudomonas putida MAS-1

Bacteriocins have been the subject of extensive research globally due to wide range applications. the aim of this research was to investigate the production of bacteriocin(s) or bacteriocin like inhibitory substance(s) by pseudomonas putida mas-1 strain. the bacteriocin produced (putidacin mas-1) was found bioactive against clinical staphylococcus aureus and enterococcus faecalis strains. bioactivity was observed by stab & overlay assay and multiwell antagonistic activity assay. putidacin mas-1 was sensitive beyond 70°c but stable at wide ph range (3 to 8). bioactivity of putidacin mas-1 was lost after treatment with trypsin and protease while partially lost after proteinase k treatment. treatment with ethanol,methanol,chloroform,acetone,tween 20 and tween 80 showed a partial decrease in bioactivity. sds had stimulatory effect on putidacin mas-1 bioactivity. edta however,showed no effect on the bacteriocin bioactivity. it was partially purified by ammonium sulphate precipitation. sds-page showed that putidacin mas-1 had 15 kda molecular weight.