Development of iodimetric redox method for routine estimation of ascorbic acid from fresh fruit and vegetables

The iodimetric method (im) is developed for rapid estimation of ascorbic acid from fresh fruit and vegetables. the efficiency of im was compared with standard dye method (dm) utilizing a variety of model solutions and aqueous extracts from fresh fruit and vegetables of different colors. the im presented consistently accurate and precise results from colorless to colored model solutions and from fruit/vegetable extracts with standard deviation (stdev) in the range of ±0.013 - ±0.405 and ±0.019 - ±0.428 respectively with no significant difference between the replicates. the dm worked also satisfactorily for colorless model solutions and extracts (stdev range ±0.235 - ±0.309) while producing unsatisfactory results (±0.464 - ±3.281) for colored counterparts. severe discrepancies/ overestimates continued to pileup (52% to 197%) estimating the nutrient from high (3.0 mg/10ml) to low (0.5 mg/10ml) concentration levels,respectively. on the basis of precision and reliability,the im technique is suggested for adoption in general laboratories for routine estimation of ascorbic acid from fruit and vegetables possessing any shade.