Quantification of pure refined olive oil adulterant in extra virgin olive oil using diamond cell ATR-FTIR spectroscopy

The present study depicts spectroscopic method development to deliver a rapid,simple and reproducible quantification of pure refined olive oil (proo) adulterant in extra virgin olive oil (evoo) using partial least square (pls) regression (statistical parameter). single bounce attenuated total reflectance (sb-atr) fourier transform infrared (ftir) was choice in the developed method. blended standards of proo and evoo were obtained by their weight by weight percentage and the values were used to construct calibration curves for quantification. the optimum regression values (i.e. >0.99) were achieved using the combined frequencies of 3105-2761,1838-1687,and 1482-440 cm-1 with regression coefficients (r2) 0.99718 and achieved residual mean square error of calibration (rmsec) 1.40% w/w. to determine the suitability of developed method principal component spectra (pcs) diagnostic was also used. the results of the present study prove that the developed methods reported in preceding studies can be good option for more rapid and accurate determination of proo adulteration in evoo.