Study on transfer rule of chemical constituents of Tianshu capsule in productive process by high-performance liquid chromatography coupled with diode-array detection and quadrupole time-of-flight tandem mass spectrometry

To develop a sensitive and accurate method for the fingerprint study and transfer rule of chemical constituents from ligusticum chuanxiong hort and gastrodia elata blume to tianshu capsule in productive process,a high performance liquid chromatography coupled with diode-array detection and electrospray ionization quadrupole time-of-flight mass spectrometry (lc/qtof-ms) method was established for analysis. the reference fingerprints of aqueous extract intermediate of medicinal material,alcohol extract intermediate of medicinal material and tianshu capsule was established. the methodology was studied and the similarity was more than 0.99. the chromatographic methods demonstrated a good precision,repeatability,stability,with relative standard deviations of less than 3% for relative retention time and relative peak area. according to these fingerprints,some chemical constituents in the fingerprints were identified or tentatively identified based on their retention time,exact molecular weight and literature. among of them 26 constituents were from ligusticum chuanxiong hort and nine components were from gastrodia elata blume. 25 out of 26 compounds had entered a transfer process and 17 compounds were transferred from intermediates to the final preparation,the tianshu capsule. thus,it is reasonable enough to use this transfer process to demonstrate the production technology. to sum up,this method is sensitive,accurate and useful,and it could provide us an approach to evaluate the production technology of tianshu capsule. � 2016,chemical society of pakistan. all rights reserved.