|
|
|
|
Fluorescence quenching dynamics and mechanism of Cry1Ab toxin from Bacillus thuringiensis by different metal ions
|
|
|
|
|
|
|
|
نویسنده
|
zhou x. ,zhang j. ,she c. ,liu h.
|
|
منبع
|
journal of the chemical society of pakistan - 2016 - دوره : 38 - شماره : 3 - صفحه:524 -532
|
|
چکیده
|
The reaction dynamics of cry1ab toxin from bacillus thuringiensis with sodium,calcium and lead ions was studied by fluorescence quenching technique. gradual quenching was observed by titration of cry1ab toxin with metal ions (na+,ca2+ or pb2+). the quenched strength of these ions in the descending order was: lead ion > calcium ion > sodium ion. the quenching equilibrium of cry1ab toxin by metal ions reached within 60 min,and the quenching dynamics of cry1ab toxin could be expressed by the elovich model. the toxin concentration,ph and temperature had influence on the quenching dynamics. the interaction between cry1ab toxin and metal ions is based on static quenching mechanism. © 2016,chemical society of pakistan. all rights reserved.
|
|
کلیدواژه
|
Cry1Ab toxin; Fluorescence spectroscopy; Metal ions; Quenching
|
|
آدرس
|
tianjin engineering and technology research center of agricultural products processing,college of food science and bioengineering,tianjin agricultural university,tianjin, China, tianjin engineering and technology research center of agricultural products processing,college of food science and bioengineering,tianjin agricultural university,tianjin, China, college of agronomy,resources and environment,tianjin agricultural university,tianjin, China, college of agronomy,resources and environment,tianjin agricultural university,tianjin, China
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Authors
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|