Antimicrobial and antioxidant activity of Citharexylum spinosum

The antimicrobial and antioxidant potential of different parts of citharexylum spinosum l. was evaluated. the antimicrobial activity was estimated by agar well diffusion method. the maximum antibacterial activity (44.5 ± 0.5 mm) was observed by methanolic bark extract against staphylococcus aureus while the minimum activity (10.5 ± 0.5 mm) was exhibited by the chloroform leaves extract against staphylococcus aureus. the highest antifungal activity (41.83 ± 0.76 mm) reported by distilled water extract of leaves against a. niger while petroleum ether extract of bark showed minimum activity (11.16 ± 0.28 mm) against a. oryzae. the most resistant value of mic was observed at concentration of 0.3125 mg/ml of methanol leaves extract against b. subtilis and p. aeruginosa. the antioxidant potential was analyzed by using five techniques included total phenolic content (tpc),total flavanoids content (tfc),abts,metal chelating activity,and dpph free radical scavenging activity. the results displayed that petroleum ether bark extract showed maximum tpc value (60.24 ± 0.03 μg/ml). petroleum ether extract of bark exhibited maximum tfc value (1350.07 ± 0.01 μg/ml). abts results showed that distilled water extract of bark exhibited maximum teac value (7.92 ± 0.06 mm). metal chelating results showed that maximum % inhibition (64.2 ± 0.05 %) was observed by distilled water extract of bark. the highest scavenging effects (82.59 ± 0.66 %) was observed by chloroform extract of leaves. the phytochemical analysis of citharexylum spinosum l showed the presence of alkaloids,tannin,terpenoids,saponins,reducing sugar,anthraquinones,cardiac glycosides and flavonoids. © 2017,chemical society of pakistan. all rights reserved.