HPLC determination of entecavir in pure,tablet dosage form and spiked plasma

Entecavir is an analogue with selective activity against hepatitis b virus. in this study,an hplc method for the determination of entecavir was developed. entecavir was eluted through c18 ods hypersil column of 150 x 4.6 mm id with 5 μm particle size using simple mobile phase of acetonitrile: 10 mm phosphate buffer (80:20) at a flow rate of 1.0 ml min-1 and eluate was detected at 218 nm. etoposide was used as an internal standard. the accuracy of the developed method was 97-99% for both with-in-batch and between-batches studies and cv (%) of < 3. the new method is highly sensitive upto 0.0097 μg ml-1. the validation results and statistical data demonstrate that the method is more sensitive,reliable and reproducible and has an importance in quality assurance of entecavir analysis and in bioequivalence studies. © 2017,chemical society of pakistan. all rights reserved.