Liquid chromatographic method for simultaneous determination of citalopram with NSAIDs in bulk drug,pharmaceutical formulation and human serum

A high performance liquid chromatographic method was developed and validated to simultaneously quantify citalopram with piroxicam,celecoxib and diclofenac sodium. chromatographic analysis was performed at ambient temperature using shimadzu shim-pack clc-ods (m) 25m column linked to a uv-visible detector adjusted at 230 nm,employing 80:20 (v/v) methanol: water (ph 3.5) as mobile phase with flow rate 1.0 ml min-1. validation was performed in the ranges 0.6-20,0.9-28,0.6-20 and 1.0-32 µg ml-1 with lowest level corresponding to detection limit 16.45,23.33,27.66 and 14.44 ng ml-1 respectively. with-in the day precision ranged from 0.14-1.67% and between-day precision from 0.40-1.50%,accuracies were 99.61-100.86%. the analytes were successfully detected without any observable interference in pharmaceutical formulation and human serum samples demonstrating effectiveness of method. © 2017 journal of the chemical society of pakistan. all rights reserved.