sertoli cell-conditioned medium restores spermatogenesis in mice induced by scrotal hyperthermia

Introduction: spermatogenesis is a complex physiological process susceptible to various influencing factors, among which scrotal hyperthermia is noteworthy. this investigation assesses the impact of conditioned medium derived from sertoli cells on spermatogenic activity within the testicular tissue of adult male mice after exposure to scrotal hyperthermia.methods: a cohort of 40 adult male nmri mice was employed in this research, and they were allocated ran-domly into four distinct groups as follows: (1) the control group comprising untreated animals, (2) the hyperthermia group subjected to scrotal hyperthermia through immersion in water at a tempera-ture of 43°c for 20 minutes, administered five times every other day, (3) the dmem group receiv-ing 10μl of dulbecco’s modified eagle medium (dmem), and (4) the sertoli cell-conditioned me-dium (sccm) group, administered 10μl of sccm. after the induction of hyperthermia, intraperito-neal injections of sccm were administered to the mice daily for 35 days. samples of sperm were gathered from the epididymal tail, and the testis tissue was subsequently harvested for histological examination and molecular analysis.results: our findings revealed that the administration of sccm yielded significant increases in the quanti-ties of sperm and germ cells compared to the other groups. additionally, the relative gene expres-sion levels of receptor tyrosine kinase (c-kit), stimulated by retinoic acid 8 (stra8), and proliferat-ing cell nuclear antigen (pcna) exhibited a substantial elevation within the sccm group compared to the other experimental groups.conclusion: the utilization of sccm holds promise as a therapeutic intervention for addressing infertility con-cerns, and it presents potential applications within the realms of the field of reproductive and re-generative medicine.