the efficacy of hypericum helianthemoides extraction with phenytoin in diabetic and non-diabetic wound healing: an experimental study in rats

Aintroduction: traditional medicine recommends herbal medicines for metabolic disorders. the present study explores the skin wound healing potential of hypericum helianthemoides (h. helianthemoides) extract in diabetic and non-diabetic rats. methods: following wound induction in diabetic (n=50) (induced by a single dose of streptozotocin) and non-diabetic (n=50) rats, h. helianthemoides extract (5% and 10%) was administered versus standard drug phenytoin (1%) and osrin to positive and sham control groups. tropical ointment therapy was applied once a day until the end of the study period (20 days). a vernier caliper (with a 0.1 mm accuracy) was used to measure the wound length at 1, 3, 7, 11, 15, and 20 days after induction. furthermore, pathological examination categorized the wound healing process into five categories: poor, mild, moderate, fair, and excellent. results: on the study’s first day, both diabetic and non-diabetic rats had the same wound area size. after 11 days, the wound area size significantly decreased in groups treated with 5% and 10% h. helianthemoides extract compared to the sham and control groups (p<0.001). hence, based on the wound pathological evaluation scale, the most frequent phenytoin and h. helianthemoides extract-treated groups were classified as moderate to excellent (p<0.05). conclusion: h. helianthemoides extract accelerates full-thickness wound healing in diabetic and non-diabetic rats.