cholic acid enhanced hypercholesterol parameters in high cholesterol diet fed sprague-dawley rats

Introduction: hypercholesterolemia is a condition in which the blood contains elevated levels of low-density lipoprotein (ldl) and non-high-density lipoprotein (hdl). there are varieties of different diets used by different laboratories as a recipe for induction with varying levels of hypercholesterolemia. this study aims to investigate the role of cholic acids in enhancing hypercholesterolemia parameters in sprague dawley rats. methods: nine sprague dawley rats (250 g ± 50 g bw) were used to investigate the most effective diet that is cost-effective for inducing hypercholesterolemia. the rats were randomly divided into 3 groups: normal diet (nd) (n=3), high cholesterol diet (hcd 1), a combination of 2% cholesterol and 0.5% cholic acid (n=3), and high hcd 2, a combination of 2% cholesterol and 30% ghee (n=3). after 4 weeks of feeding, blood samples were collected for lipid profiling, which included total cholesterol (tc), triglycerides (tg), high-density lipoprotein (hdl), and low-density lipoprotein (ldl). the liver, kidney, and brain were removed for histopathological examination using hematoxylin and eosin (h&e) staining. results: the lipid profile measurements show significant differences between the hcd 1 group for total cholesterol, ldl cholesterol, non-hdl cholesterol, and total cholesterol/hdl ratio compared to the normal group. hcd 2 shows no significant changes in lipid profiles compared to the normal group. conclusion: cholic acid helps in the absorption of cholesterol and enhances the hypercholesterol parameters in diet-induced sd rats based on lipid profile analysis and histology of the liver and kidney.