effect of thioflavin-t on adipokine hormones and fatty liver in obese male nmri mice fed a high-fat diet

Introduction: the objective of the study was to find out the influence of thioflavin-t (tht) on obesity and fatty liver by investigating the adipokine hormones, and insulin serum level of male nmri mice which were exposed to a high-fat diet (hfd). methods: 50 adult male nmri mice were separated into five groups: n=10. the control group was given a standard diet at twelve-week intervals. the sham group was nourished with hfd that lasted for 8 weeks, afterwards, the group received a standard diet and solvent water (0.5ml) by gavage (4 weeks). the experimental groups 1-3 were nourished with hfd (4% cholesterol, 60% fat) eight-week period. then, the treatment period started in experimental groups by receiving a normal diet in addition to tht with three doses (5,10 and 15 mg/kg, 0.5ml), via gavage (4 weeks). results: hfd contributed to a substantial reduction in serum adiponectin levels and increased leptin serum in the sham group opposite to the control group (p<0.001). however, the concentration of both adipokine hormones was significantly modified under the treatment of tht in a dose-dependent manner. insulin serum increased in the sham group significantly (p< 0.001), meanwhile, a significant decrease was shown in experimental groups 2, and 3 than in the sham group (p<0.01). tht also reduced homa-ir in experimental groups. the introduction of tht in varying doses led to the induction of polymorphonuclear cells in the liver tissue. conclusion: our findings propose that tht can affect liver function and body weight by modulating the serum levels of adipokine hormones besides decreasing the level of insulin and homa-ir in mice fed with hfd.