omega-3 pufas counteracts high fructose diet-induced gonadal impairment via fortified antioxidant defense

Introduction: the consumption of fructose sweeteners as an alternative to high-calorie diets has been linked to various metabolic diseases due to increased oxidative stress. this study investigated changes in testicular and ovarian functions following omega-3 polyunsaturated fatty acid supplementation (ω-3 pufas) in rats fed a high fructose diet (hfd).methods: forty wistar rats were equally and randomly distributed into four groups. each group was further subdivided by gender, with an equal number of animals in each sub-group. group a assigned as control, while groups b and c were administered hfd containing 10% and 20% v/v fructose solution, respectively. group d rats were provided 20% v/v hfd and ω-3 pufas. results: after six weeks of consumption, hfd resulted in reduced semen quality and gonadal reproductive hormone levels, while increasing testicular and ovarian oxidative stress. ω-3 pufas improved semen quality, reproductive hormone concentrations, and antioxidant defense system.conclusion: hfd impairs gonadal function, however, dietary supplementation with ω-3 pufas improves gonadal functions by enhancing the antioxidant defense mechanism.