Effect of Cell-Free Supernatant of Bifidobacterium Bifidum Combined With Chitosan Biodegradable Film on Full Thickness Wound Healing in Rats

Introduction: wound healing is one of the most critical issues human has been faced since the beginning of creation. biodegradable polymers are of particular importance. in this study, cell-free supernatant (cfs) of bifidobacterium bifidum combined with chitosan (ch) film was evaluated as a wound dressing. methods: biodegradable films (ch and cfs/ch), as a novel wound dressing, were prepared. for the evaluation of dressing efficacy, 45 male wistar rats weighing 200-250 g were randomly divided into 3 groups: negative control (without wound treatment), positive control (wound treatment by ch film), and probiotic (wound treatment by cfs/ch film). one full thickness wound was created on the dorsal area of the animals. the wound in positive control and probiotic groups were immediately covered by ch and cfs/ch dressing, respectively. wound healing process was evaluated by macroscopic observation and histological analysis. during the treatment the expression of il-1, tgf-b and il-6 were assayed by qrt-pcr. results: our results showed different infiltration patterns of macrophages, fibroblasts, and neutrophils in cfs/ch treated group. enhanced disposition of collagen and elastin caused improvement of wound healing process by the film. based on the gene expression results, use of cfs/ch film caused improvement in wound healing kinetic. conclusion: the biodegradable film based on chitosan and cfs of b. bifidum improves the wound healing process.