Neuroprotective Effects of Caffeine Against Beta-Amyliod Neurotoxicity: the Involvement of Glycogen Synthase Kinase-3β Protein

Introduction: the reduction of glycogen synthase kinase-3β protein level may correlate to the neuroprotective effects of antioxidant agents like caffeine. therefore, we aimed to evaluate the impact of gsk-3β protein on neuroprotective effects of caffeine in the shsy5y cells exposed to beta-amyloid. methods: we incubated shsy5ycells with beta-amyloid 25–35 and caffeine (0.6 and 1mm) for 24h. cell viability was determined using mtt test. we used the western blotting technique to measure the glycogen synthase kinase-3β and phosphorylated glycogen synthase kinase-3β protein levels. results: caffeine (0.6 and 1mm) diminished beta-amyloid neurotoxicity and attenuated the beta-amyloid effects on the glycogen synthase kinase-3β protein level in a neuronal culture. conclusion: caffeine neuroprotective effects against beta-amyloid may correlate to glycogen synthase kinase-3β protein.