CDNA Cloning and expression analysis of three components of scf coi1 complex in hevea brasiliensis

To gain a deeper understanding ofjasmonate signaling in specific tissues,three of full-length cdnas,referred to as hbcul1,hbskp1and hbrbx1 were isolated from laticifers of rubber tree by the rapid amplification of cdna ends (race) method and analysed by sequence analysis and real-time rt-pcr. the putative proteins,hbcul1,hbskp1 and hbrbx1,had specific domains of cullin superfamily and cullin nedd8,skp1-poz and skp1,and ring superfamily,respectively. hbcul1 and hbskp1 were most related to atcul1 and atask1 among the members of cullins and asks from arabidopsis,respectively. methyl jasmonate was more effective than ethylene on up-regulating the expression of hbcul1,hbskp1 and hbrbx1. these data suggest that hbcul1,hbskp1 and hbrbx1 may be the invariant components of scf coi1 complex ofjasmonate signaling in laticifers of rubber tree.