Cell viability assay guided fractionation of natural rubber latex sera

Natural rubber latex sera have been investigated for anti-proliferative property on cancer cell lines. the present report describes fractionation of latex b- and c-serum using cell viability assay (anti-proliferative activity) as a guide to narrow down on the active constituents. dialysis of b- and c-serum against distilled water caused some of its constituents to precipitate,then a brief centrifugation separated their fractions - dialysed b-serum precipitate (dbp),dialysed b-serum supernatant (dbs),dialysed c-serum precipitate dcp and dialysed c-serum supernatant (dcs). boiling of b- and c-serum resulted in extensive precipitation; a brief centrifugation separated the boiled b- and c-serum precipitate from their supernatant. cell viability assay performed on two human cancer-origin cell lines revealed a marked increase in anti-proliferative activity in the dialysed c-serum fractions and in dbp but diminished in dbs as well as in the boiled b- and c-serum fractions. chromatographic separation of dbp,dcp and dcs followed by cell viability assay will shed light if sub-fractions with further improved anti-proliferative activity are attainable.