Proteins of dialysed C-serum supernatant sub-fractions elicit anti-proliferative activity on human cancer-origin cells

Dialysed latex c-serum fractions have been investigated for anti-proliferative activity against human cancer origin cell lines. the present report describes further fractionation of dialysed c-serum supernatant (dcs) and analysis of the sub-fractions that elicit anti-proliferative activity on hepg2 and mda-mb231,the test cancer origin cell lines. dcs was fractionated through a sephacryl s-200 column,and the eluents were pooled into four sequential sub-fractions,named dcs-f1,-f2,-f3 and -f4. cell viability assay revealed that anti-proliferative activity was confined to dcs-f2 and -f3. furthermore an improved anti-proliferative activity was discerned in the recombined active dcs sub-fractions. dcs-f2 and -f3 were subjected to two dimensional polyacrylamide gel electrophoresis,subsequent maldi-tof/tof analysis and database searches revealed the presence of a number of proteins that have been shown to be implicated in anti-cancer activity.