Gene silencing of tead3 abrogates radiation-induced adaptive response in cultured mouse limb bud cells

There is a crucial need to better understand the effects of low-doses of ionizing radiation in fetal models. radiation-induced adaptive response (ar) was described in mouse embryos pre-exposed in utero to low-doses of x-rays,which exhibited lower apoptotic levels in the limb bud. we previously described ar-specific gene modulations in the mouse embryo. in this study,we evaluated the role of three candidate genes in the apoptotic ar in a micromass culture of limb bud cells: csf1,cacna1a and tead3. gene silencing of these three genes abrogated ar. knowing that tead3 protein levels are significantly higher in adapted cells and that yap/taz/tead are involved in the control of cell proliferation and apoptosis,we suggest that modulation of tead3 could play a role in the induction of ar in our model,seen as a reduction of radiation-induced apoptosis and a stimulation of proliferation and differentiation in limb bud cells.