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Ascorbic acid enhances radiation-induced apoptosis in an HL60 human leukemia cell line
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نویسنده
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shinozaki k. ,hosokawa y. ,hazawa m. ,kashiwakura i. ,okumura k. ,kaku t. ,nakayama e.
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منبع
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journal of radiation research - 2011 - دوره : 52 - شماره : 2 - صفحه:229 -237
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چکیده
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This study was conducted to examine the utility of the combined use of ascorbic acid (asa) and radiation in clinical applications. we investigated cell survival,dna fragmentation,and caspase activation after x-ray irradiation and asa treatment of human leukemia hl60 cells. the number of living cells decreased after combined x-ray irradiation and asa treatment (2 gy + 5 mm) in comparison with that after x-ray irradiation (2 gy) or asa treatment (5 mm) alone. dna fragmentation was more in the cells subjected to combined x-ray irradiation and asa treatment than in those subjected to x-ray irradiation alone. caspase-3,caspase-8,and caspase-9 were highly activated following combined x-ray irradiation and asa treatment,but caspase-8 activity was not markedly increased after x-ray irradiation alone. bax levels in the mitochondrial membrane fractions were increased after asa treatment alone and after combined x-ray irradiation and asa treatment. however,there was no apparent increase in the bax levels after x-ray irradiation treatment alone. thus,this study confirmed that supplementing x-ray irradiation with asa treatment results in increased apoptosis in hl60 cells. with regard to the apoptosis-inducing factors,we hypothesized that bax and caspase-8 were activated after combined x-ray irradiation and asa treatment compared with either treatment alone.
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آدرس
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division of oral and maxillofacial radiology,school of dentistry,health sciences university of hokkaido,1757 kanazawa,ishikari-tobetsu,hokkaido 061-0293, Japan, department of radiological life sciences,division of medical life sciences,hirosaki university,66-1 honcho,hirosaki,aomori 036-8564, Japan, department of radiological life sciences,division of medical life sciences,hirosaki university,66-1 honcho,hirosaki,aomori 036-8564, Japan, department of radiological life sciences,division of medical life sciences,hirosaki university,66-1 honcho,hirosaki,aomori 036-8564, Japan, division of reconstructive surgery for oral and maxillofacial region,school of dentistry,health sciences university of hokkaido,1757 kanazawa,ishikari-tobetsu,hokkaido 061-0293, Japan, division of clinical oral pathology,department of human biology and pathophysiology,school of dentistry,health sciences university of hokkaido,1757 kanazawa,ishikari-tobetsu,hokkaido 061-0293, Japan, division of oral and maxillofacial radiology,school of dentistry,health sciences university of hokkaido,1757 kanazawa,ishikari-tobetsu,hokkaido 061-0293, Japan
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Authors
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