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   Ascorbic acid enhances radiation-induced apoptosis in an HL60 human leukemia cell line  
   
نویسنده shinozaki k. ,hosokawa y. ,hazawa m. ,kashiwakura i. ,okumura k. ,kaku t. ,nakayama e.
منبع journal of radiation research - 2011 - دوره : 52 - شماره : 2 - صفحه:229 -237
چکیده    This study was conducted to examine the utility of the combined use of ascorbic acid (asa) and radiation in clinical applications. we investigated cell survival,dna fragmentation,and caspase activation after x-ray irradiation and asa treatment of human leukemia hl60 cells. the number of living cells decreased after combined x-ray irradiation and asa treatment (2 gy + 5 mm) in comparison with that after x-ray irradiation (2 gy) or asa treatment (5 mm) alone. dna fragmentation was more in the cells subjected to combined x-ray irradiation and asa treatment than in those subjected to x-ray irradiation alone. caspase-3,caspase-8,and caspase-9 were highly activated following combined x-ray irradiation and asa treatment,but caspase-8 activity was not markedly increased after x-ray irradiation alone. bax levels in the mitochondrial membrane fractions were increased after asa treatment alone and after combined x-ray irradiation and asa treatment. however,there was no apparent increase in the bax levels after x-ray irradiation treatment alone. thus,this study confirmed that supplementing x-ray irradiation with asa treatment results in increased apoptosis in hl60 cells. with regard to the apoptosis-inducing factors,we hypothesized that bax and caspase-8 were activated after combined x-ray irradiation and asa treatment compared with either treatment alone.
آدرس division of oral and maxillofacial radiology,school of dentistry,health sciences university of hokkaido,1757 kanazawa,ishikari-tobetsu,hokkaido 061-0293, Japan, department of radiological life sciences,division of medical life sciences,hirosaki university,66-1 honcho,hirosaki,aomori 036-8564, Japan, department of radiological life sciences,division of medical life sciences,hirosaki university,66-1 honcho,hirosaki,aomori 036-8564, Japan, department of radiological life sciences,division of medical life sciences,hirosaki university,66-1 honcho,hirosaki,aomori 036-8564, Japan, division of reconstructive surgery for oral and maxillofacial region,school of dentistry,health sciences university of hokkaido,1757 kanazawa,ishikari-tobetsu,hokkaido 061-0293, Japan, division of clinical oral pathology,department of human biology and pathophysiology,school of dentistry,health sciences university of hokkaido,1757 kanazawa,ishikari-tobetsu,hokkaido 061-0293, Japan, division of oral and maxillofacial radiology,school of dentistry,health sciences university of hokkaido,1757 kanazawa,ishikari-tobetsu,hokkaido 061-0293, Japan
 
     
   
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