>
Fa   |   Ar   |   En
   Characterization and radio-resistant function of manganese superoxide dismutase of rubrobacter radiotolerans  
   
نویسنده terato h. ,suzuki k. ,nishioka n. ,okamoto a. ,shimazaki-tokuyama y. ,inoue y. ,saito t.
منبع journal of radiation research - 2011 - دوره : 52 - شماره : 6 - صفحه:735 -742
چکیده    Rubrobacter radiotolerans is the most radio-resistant eubacterium without spore-formation in the life cycle,and its d37 is 16,000 gy against gamma-rays. to understand the molecular mechanism of the high radio-resistance,we purified and characterized superoxide dismutase (sod) of this organism as enzymatic radical scavenger,and then analyzed its genetic information. the purified sod protein formed homo-tetramerization of 24,000 da-monomer,while maintaining its enzymatic activity against potassium cyanide and hydrogen peroxide. we obtained a partial amino acid sequence of the protein and cloned the gene from it. sequence analysis of the cloned gene indicated that the protein showed a similarity to other bacterial manganese sods (mn-sods). sequencing for adjacent regions of the gene showed that the gene had promoter elements with an open reading frame for putative pas/pac sensor protein at the 5'-adjacent region. introduction of the gene into escherichia coli cells lacking intrinsic sod genes restored the cellular enzymatic activity and resistance to methyl viologen,indicating the gene at work. a mutant cell harboring this gene also became resistant against gamma-rays. the present results suggest that the protein in question is the mn-sod of r. radiotolerans,a good candidate as a radio-protection factor for this bacterial radio-resistance.
کلیدواژه Radical scavenger; Radio-resistance; Rubrobacter radiotolerans; Superoxide dismutase
آدرس analytical research center for experimental sciences,saga university,5-1-1 nabeshima,saga 849-8501, Japan, faculty of engineering,kinki university,1 takaya umenobe,higashi-hiroshima 739-2116, Japan, faculty of engineering,kinki university,1 takaya umenobe,higashi-hiroshima 739-2116, Japan, faculty of engineering,kinki university,1 takaya umenobe,higashi-hiroshima 739-2116, Japan, analytical research center for experimental sciences,saga university,5-1-1 nabeshima,saga 849-8501, Japan, analytical research center for experimental sciences,saga university,5-1-1 nabeshima,saga 849-8501, Japan, research reactor institute,kyoto university,2 asashiro-nishi,kumatori 590-0494, Japan
 
     
   
Authors
  
 
 

Copyright 2023
Islamic World Science Citation Center
All Rights Reserved