|
|
NEIL1 mRNA splicing variants are expressed in normal mouse organs
|
|
|
|
|
نویسنده
|
yamamoto r. ,yamamoto m. ,kusaka h. ,masatsugu h. ,matsuyama s. ,tajima t. ,ide h. ,kubo k.
|
منبع
|
journal of radiation research - 2012 - دوره : 53 - شماره : 2 - صفحه:234 -241
|
چکیده
|
Oxidized pyrimidines are mainly repaired by base excision repair,which is initiated by damagespecific dna glycosylases. neil1,the mammalian homolog of escherichia coli endonuclease viii and a major dna glycosylase,initiates repair of oxidized pyrimidines. here,we investigated the expression of two putative variant mouse neil1 (mneill) mrnas-variant 1 (neil 1 protein mrna; bc043297 in the ncbi database) and variant 2 (unnamed protein mrna; ak040802 in the ncbi database)-in normal mouse organs. reverse transcription-pcr showed that both mrnas were expressed in total rna samples from 9 organs. immunoblot analysis of a nuclear extract from normal mouse liver revealed three bands corresponding to full-length mneill protein and the two predicted variant proteins. however,neither variant protein,which included an n-terminal enzymatic activity domain deduced from the mrna variants,were enzymatically active under multiple reaction conditions when expressed as his-tagged recombinant proteins. nevertheless,recombinant variant 1 protein influenced mneill activity,while recombinant variant 2 protein had no influence. these results suggest that mneill mrna variants are expressed in a variety of organs in normal mice and that variant 1 protein may regulate mneill activity.
|
کلیدواژه
|
BER; Glycosylase; Mouse; NEILI; Splicing variant
|
آدرس
|
department of advanced pathobiology,graduate school of life and environmental sciences,osaka prefecture university,1-58 rinku ourai kita,izumisano,osaka 598-8531, Japan, department of advanced pathobiology,graduate school of life and environmental sciences,osaka prefecture university,1-58 rinku ourai kita,izumisano,osaka 598-8531, Japan, department of advanced pathobiology,graduate school of life and environmental sciences,osaka prefecture university,1-58 rinku ourai kita,izumisano,osaka 598-8531, Japan, department of advanced pathobiology,graduate school of life and environmental sciences,osaka prefecture university,1-58 rinku ourai kita,izumisano,osaka 598-8531, Japan, department of advanced pathobiology,graduate school of life and environmental sciences,osaka prefecture university,1-58 rinku ourai kita,izumisano,osaka 598-8531, Japan, department of infectious diseases control,graduate school of life and environmental sciences,osaka prefecture university,1-58 rinku ourai kita,izumisano,osaka 598-8531, Japan, department of mathematical and life sciences,graduate school of science,hiroshima university,1-3-1 kagamiyama,higashi-hiroshima,hiroshima 739-8526, Japan, department of advanced pathobiology,graduate school of life and environmental sciences,osaka prefecture university,1-58 rinku ourai kita,izumisano,osaka 598-8531, Japan
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Authors
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|