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Beclinl-induced autophagy abrogates radioresistance of lung cancer cells by suppressing osteopontin
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نویسنده
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chang s.-h. ,minai-tehrani a. ,shin j.-y. ,park s. ,kim j.-e. ,yu k.-n. ,hong s.-h. ,hong c.-m. ,lee k.-h. ,beck jr. g.r. ,cho m.-h.
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منبع
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journal of radiation research - 2012 - دوره : 53 - شماره : 3 - صفحه:422 -432
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چکیده
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Osteopontin (opn) serves as an indicator of resistance to radiotherapy. however,the role of opn in the development of acquired radioresistance in human lung cancer cells has not yet been fully elucidated. therefore,the potential importance of opn as a marker of lung cancer with a potential significant role in the development of radioresistance against repeated radiotherapy has prompted us to define the pathways by which opn regulates lung cancer cell growth. in addition,autophagy has been reported to play a key role in the radiosensitization of cancer cells. here,we report that increased opn expression through induction of nuclear p53 following irradiation was inhibited by exogenous beclin-1 (becn1). our results clearly show that becn1 gene expression led to induction of autophagy and inhibition of cancer cell growth and angiogenesis. our results suggest that the induction of autophagy abrogated the radioresistance of the cancer cells. interestingly,we showed that knockdown of opn by lentivirus-mediated shrna induced the autophagy of human lung cancer cell. taken together,these results suggest that opn and becn1 can be molecular targets for overcoming radioresistance by controlling autophagy.
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کلیدواژه
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Angiogenesis; Beclin-l(BECNl); Gamma-irradiation; Osteopontin(OPN); P53
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آدرس
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laboratory of toxicology,college of veterinary medicine,seoul national university,seoul 151-742, South Korea, laboratory of toxicology,college of veterinary medicine,seoul national university,seoul 151-742, South Korea, laboratory of toxicology,college of veterinary medicine,seoul national university,seoul 151-742, South Korea, laboratory of toxicology,college of veterinary medicine,seoul national university,seoul 151-742, South Korea, laboratory of toxicology,college of veterinary medicine,seoul national university,seoul 151-742,south korea,department of nanofusion technology,graduate school of convergence science and technology,seoul national university,seoul 151-742, South Korea, laboratory of toxicology,college of veterinary medicine,seoul national university,seoul 151-742, South Korea, laboratory of toxicology,college of veterinary medicine,seoul national university,seoul 151-742, South Korea, division of medical device management,osong health technology administration complex,cheongwon 363-700, South Korea, laboratory of radiation molecular oncology,korea institute of radiological and medical sciences,seoul 139-709, South Korea, division of endocrinology,metabolism and lipids,emory university school of medicine,atlanta,ga 30322, United States, laboratory of toxicology,college of veterinary medicine,seoul national university,seoul 151-742,south korea,department of nanofusion technology,graduate school of convergence science and technology,seoul national university,seoul 151-742,south korea,graduate group of tumor biology,seoul national university,seoul 151-742,south korea,center for food safety and toxicology,seoul national university,national institute of food and drug safety,seoul 151-742,south korea,advanced institute of convergence technology,seoul national university,suwon 443-270, South Korea
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Authors
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