Protective activity of a novel resveratrol analogue,HS-1793,against DNA damage in 137Cs-irradiated CHO-K1 cells

Resveratrol has received considerable attention as a polyphenol with anti-oxidant,anti-carcinogenic,and anti-inflammatory effects. radiation is an important component of therapy for a wide range of malignant conditions. however,it causes damage to normal cells and,hence,can result in adverse side effects. this study was conducted to examine whether hs-1793,a novel resveratrol analogue free from the restriction of metabolic instability and the high dose requirement of resveratrol,induces a protective effect against radiation-induced dna damage. hs-1793 effectively scavenged free radicals and inhibited radiation-induced plasmid dna strand breaks in an in vitro assay. hs-1793 significantly decreased reactive oxygen species and cellular dna damage in 2 gy-irradiated chinese hamster ovary (cho)-k1 cells. in addition,hs-1793 dose-dependently reduced the levels of phosphorylated h2ax in irradiated cho-k1 cells. these results indicate that hs-1793 has chemical radioprotective activity. glutathione levels and superoxide dismutase activity in irradiated cho-k1 cells increased significantly following hs-1793 treatment. the enhanced biological anti-oxidant activity and chemical radioprotective activity of hs-1793 maintained survival of irradiated cho-k1 cells in a clonogenic assay. therefore,hs-1793 may be of value as a radioprotector to protect healthy tissue surrounding tumor cells during radiotherapy to obtain better tumor control with a higher dose. © 2014 the author 2014. published by oxford university press on behalf of the japan radiation research society and japanese society for radiation oncology.