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Radioprotective effect of epicatechin in cultured human fibroblasts and zebrafish
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نویسنده
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shin h.a. ,shin y.s. ,kang s.u. ,kim j.h. ,oh y.-t. ,park k.h. ,lee b.h. ,kim c.-h.
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منبع
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journal of radiation research - 2014 - دوره : 55 - شماره : 1 - صفحه:32 -40
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چکیده
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Radiation-induced normal cell damage limits the delivery of high-dose radiation to targeted cancer. this study investigated the effect of epicatechin (ec),a minor component of green tea extracts,on radiation-induced cellular damage in vitro in primary cultured human fibroblasts and in vivo in a zebrafish model. cell viability,proliferation and wound-healing efficacy,mitochondrial membrane potential,and reactive oxygen species (ros) generation as well as changes in the signaling pathway related to apoptosis were investigated in fibroblasts. the therapeutic effects of ec were explored in a zebrafish model. ec increased clonogenic survival and restored the migration ability of the fibroblasts after irradiation. ec inhibited radiation-induced ros generation,mitochondrial dysfunction and cell death. ec significantly reduced the expression of p-jnk,p-38,and cleaved caspase-3 compared with their significant increase after radiation treatment. ec attenuated the radiation-induced embryotoxicity in a zebrafish model. these results suggest that ec represents an effective means of reducing cellular damage and facilitating wound healing after radiation exposure. © 2013 the author.
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کلیدواژه
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epicatechin; fibroblast; radiation; radioprotection; zebrafish
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آدرس
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department of otorhinolaryngology-head and neck surgery,national health insurance corporation ilsan hospital,100 ilsan street,ilsandong-gu,goyang,410-719, South Korea, department of otolaryngology,school of medicine,ajou university,164 worldcup street,wonchon-dong,yeongtong-gu,suwon,442-749, South Korea, department of otolaryngology,school of medicine,ajou university,164 worldcup street,wonchon-dong,yeongtong-gu,suwon,442-749, South Korea, department of pathology,school of medicine,ajou university,164 worldcup street,wonchon-dong,yeongtong-gu,suwon,442-749, South Korea, department of radiation oncology,school of medicine,ajou university,164 worldcup street,wonchon-dong,yeongtong-gu,suwon,442-749, South Korea, department of otolaryngology,school of medicine,ajou university,164 worldcup street,wonchon-dong,yeongtong-gu,suwon,442-749, South Korea, department of otolaryngology,school of medicine,ajou university,164 worldcup street,wonchon-dong,yeongtong-gu,suwon,442-749, South Korea, department of otolaryngology,school of medicine,ajou university,164 worldcup street,wonchon-dong,yeongtong-gu,suwon,442-749, South Korea
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Authors
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