Dose-and time-dependent gene expression alterations in prostate and colon cancer cells after in vitro exposure to carbon ion and X-irradiation

Hadrontherapy is an advanced form of radiotherapy that uses beams of charged particles (such as protons and carbon ions). compared with conventional radiotherapy,the main advantages of carbon ion therapy are the precise absorbed dose localization,along with an increased relative biological effectiveness (rbe). this high ballistic accuracy of particle beams deposits the maximal dose to the tumor,while damage to the surrounding healthy tissue is limited. currently,hadrontherapy is being used for the treatment of specific types of cancer. previous in vitro studies have shown that,under certain circumstances,exposure to charged particles may inhibit cell motility and migration. in the present study,we investigated the expression of four motility-related genes in prostate (pc3) and colon (caco-2) cancer cell lines after exposure to different radiation types. cells were irradiated with various absorbed doses (0,0.5 and 2 gy) of accelerated 13c-ions at the ganil facility (caen,france) or with x-rays. clonogenic assays were performed to determine the rbe. rt-qpcr analysis showed dose- and time-dependent changes in the expression of ccdc88a,fn1,myh9 and rock1 in both cell lines. however,whereas in pc3 cells the response to carbon ion irradiation was enhanced compared with x-irradiation,the effect was the opposite in caco-2 cells,indicating cell-type-specific responses to the different radiation types. © the author 2014. published by oxford university press on behalf of the japan radiation research society and japanese society for radiation oncology.