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Effects of Chk1 inhibition on the temporal duration of radiation-induced G2 arrest in HeLa cells
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نویسنده
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nahar k. ,goto t. ,kaida a. ,deguchi s. ,miura m.
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منبع
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journal of radiation research - 2014 - دوره : 55 - شماره : 5 - صفحه:1021 -1027
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چکیده
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Chk1 inhibitor acts as a potent radiosensitizer in p53-deficient tumor cells by abrogating the g2/m checkpoint. however,the effects of chk1 inhibitor on the duration of g2 arrest have not been precisely analyzed. to address this issue,we utilized a cell-cycle visualization system,fluorescent ubiquitination-based cell-cycle indicator (fucci),to analyze the change in the first green phase duration (fgpd) after irradiation. in the fucci system,g1 and s/g2/m cells emit red and green fluorescence,respectively; therefore,g2 arrest is reflected by an elongated fgpd. the system also allowed us to differentially analyze cells that received irradiation in the red or green phase. cells irradiated in the green phase exhibited a significantly elongated fgpd relative to cells irradiated in the red phase. in cells irradiated in either phase,chk1 inhibitor reduced fgpd almost to control levels. the results of this study provide the first clear information regarding the effects of chk1 inhibition on radiation-induced g2 arrest,with special focus on the time dimension. © 2014 the author.
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کلیدواژه
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cell cycle; Chk1; Chk1 inhibitor; fluorescent ubiquitination-based cell-cycle indicator (Fucci); G2 arrest; radiation
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آدرس
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section of oral radiation oncology,department of oral health sciences,tokyo medical and dental university,1-5-45 yushima,bunkyo-ku,tokyo,113-8549, Japan, section of oral radiation oncology,department of oral health sciences,tokyo medical and dental university,1-5-45 yushima,bunkyo-ku,tokyo,113-8549, Japan, section of oral radiation oncology,department of oral health sciences,tokyo medical and dental university,1-5-45 yushima,bunkyo-ku,tokyo,113-8549, Japan, section of oral radiation oncology,department of oral health sciences,tokyo medical and dental university,1-5-45 yushima,bunkyo-ku,tokyo,113-8549, Japan, section of oral radiation oncology,department of oral health sciences,tokyo medical and dental university,1-5-45 yushima,bunkyo-ku,tokyo,113-8549, Japan
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Authors
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