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Abundance of BER-related proteins depends on cell proliferation status and the presence of DNA polymerase β
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نویسنده
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yamamoto m. ,yamamoto r. ,takenaka s. ,matsuyama s. ,kubo k.
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منبع
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journal of radiation research - 2015 - دوره : 56 - شماره : 4 - صفحه:607 -614
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چکیده
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In mammalian cells,murine n-methylpurine dna glycosylase (mpg) removes bases damaged spontaneously or by chemical agents through the process called base excision repair (ber). in this study,we investigated the influence of pol β deficiency on mpg-initiated ber efficiency and the expression levels of ber-related proteins in log-phase and growth-arrested (g0) mouse embryonic fibroblasts (mefs). g0 wild-type (wt) or pol β-deficient (pol β-ko) cells showed greater resistance to methyl methanesulfonate than did log-phase cells,and repair of methylated bases was less efficient in the g0 cells. apex1 mrna expression was significantly lower in pol β-ko or g0 wt mefs than in log-phase wt mefs. moreover,although mpg mrna levels did not differ significantly among cell types,mpg protein levels were significantly higher in log-phase wt cells than in log-phase pol β-ko cells or either type of g0 cells. additionally,proliferating cell nuclear antigen protein levels were also reduced in log-phase pol β-ko cells or either type of g0 cells. these results indicated that mpg-initiated ber functions mainly in proliferating cells,but less so in g0 cells,and that pol β may be involved in regulation of the amount of intracellular repair proteins. © the author 2015.
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کلیدواژه
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BER; Cell proliferation; Mouse; MPG; POL β
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آدرس
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department of advanced pathobiology,graduate school of life and environmental sciences,osaka prefecture university,1-58 rinku,ourai-kita,izumisano,osaka,598-8531, Japan, department of advanced pathobiology,graduate school of life and environmental sciences,osaka prefecture university,1-58 rinku,ourai-kita,izumisano,osaka,598-8531, Japan, department of structural and functional biosciences for animals,graduate school of life and environmental sciences,osaka prefecture university,1-58 rinku,ourai-kita,izumisano,osaka,598-8531, Japan, department of advanced pathobiology,graduate school of life and environmental sciences,osaka prefecture university,1-58 rinku,ourai-kita,izumisano,osaka,598-8531, Japan, department of advanced pathobiology,graduate school of life and environmental sciences,osaka prefecture university,1-58 rinku,ourai-kita,izumisano,osaka,598-8531, Japan
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Authors
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