Identification of Wheat d-Cysteine Desulfhydrase (TaD-CDes) Required for Abscisic Acid Regulation of Seed Germination, Root Growth, and Stomatal Closure in Arabidopsis

cysteine desulfhydrase (cdes) can catalyze the degradation of cysteine producing hydrogen sulfide. in this study, d-cysteine desulfhydrase from wheat (tad-cdes) was cloned and overexpressed in arabidopsis thaliana. the physiological effects of tad-cdes were determined by investigating seed germination, root growth, stomatal closure, and drought resistance in the tad-cdes plants. results showed that, compared with wild-type plants (wt), seed germination, root growth, and stomatal closure of the tad-cdes plants were more sensitive to aba, resulting from up-regulation of aba-responsive genes (such as pyr1, abi1, abi2, hab1, hab2, snrk2, abf2, and abf4). moreover, although tad-cdes mediated aba-induced stomatal closure, tad-cdes-overexpressing plants did not show higher drought resistance than wt, which might be attributed to their increased stomatal densities.