Interleukin 1β-induced synthesis and secretion of prostaglandin E2 in the porcine uterus during various periods of pregnancy and the estrous cycle

Peri-implantation porcine embryos express interleukin-1β (il-1β),which could affect uterine activity during early pregnancy. in vitro studies were conducted to determine if il-1β stimulates secretion of pge2 and expression of cyclooxygenase-2 (cox-2) mrna and microsomal pge synthase-1 (mpges-1) mrnain uterine tissues harvested from pigs on days 10 to 11,12 to 13 and 15 to 16 of pregnancy and the estrous cycle. il-1β (10 ng/ml) increased pge2 secretion and mpges-1 mrna expression in uterine tissues isolated from pigs between days 10 to 13 of pregnancy and the estrous cycle. il-1β stimulated pge2 and mpges-1 mrna expression only in cyclic uterine tissues on days 15 to 16. interleukin- 1β increased cox-2 mrna expression in the endometrial tissues of pregnant and cyclic pigs harvested on days 10 to 13. it stimulated cox-2 expression in pregnant pigs' myometrial tissues on days 10 to 11,and on days 15 to 16 in tissues from both pregnant and cyclic pigs. the uterine secretion of pge2 in response to il-1β was determined by local intrauterine concentrations of p4 and e2. this study demonstrates that il-1β activates expression of mpges-1 mrna in uterine tissues to stimulate synthesis and secretion of pge2 on days 10 to 13 of both pregnancy and the estrous cycle. the profile of cox-2 mrna expression in the myometrium differs from its profile in the endometrium. this work provides new insight on the role of il-1β in pge2 production to overcome luteolysis in pregnant pigs.