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Protective effect of grape seed and skin extract on garlic-induced erythrocyte oxidative stress
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نویسنده
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hamlaoui s. ,mokni m. ,limam n. ,zouaoui k. ,ben rayana m.c. ,carrier a. ,limam f. ,amri m. ,marzouki l. ,aouani e.
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منبع
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journal of physiology and pharmacology - 2012 - دوره : 63 - شماره : 4 - صفحه:381 -388
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چکیده
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High garlic dose could exert adverse health properties and grape seed and skin extract (gsse) exhibit a variety of beneficial effects,even at high dose. in the present study we evaluated the toxic effect of high garlic dose treatment on antioxidant status of the blood compartment and the protective effect of gsse. rats were intraperitoneally (i.p.) administered either with garlic extract (5 g/kg bw) or gsse (500 mg/kg bw) or a combination of garlic and gsse at the same doses daily during one month. plasma parameters and erythrocytes antioxidant status were evaluated. data confirmed that high garlic dose induced anemia and a pro-oxidative state into erythrocytes characterized by increased malondialdehyde (mda),carbonyl protein and antioxidant enzyme activities as catalase (cat),peroxidase (pod) and superoxide dismutase (sod). garlic also elevated intracellular hydrogen peroxide (h2o2) and free iron whereas gsse treatment counteracted almost all garlic deleterious effects. in conclusion,high garlic dose induced a pro-oxidative state into erythrocytes via the fenton reaction between h2o2 and free iron,and gsse exerted antioxidant properties.
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کلیدواژه
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Antioxidant activity; Calcium; Erythrocytes; Garlic; Grape seed and skin extract; Hydrogen peroxide; Iron; Lipoperoxidation; Oxidative stress; Protein carbonylation
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آدرس
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laboratoire de neurophysiologie fonctionnelle et pathologies,département des sciences biologiques,faculté des sciences de tunis,tunis, Tunisia, laboratoire de neurophysiologie fonctionnelle et pathologies,département des sciences biologiques,faculté des sciences de tunis,tunis, Tunisia, laboratoire de neurophysiologie fonctionnelle et pathologies,département des sciences biologiques,faculté des sciences de tunis,tunis, Tunisia, laboratoire de biologie clinique,institut de nutrition de tunis,tunis, Tunisia, laboratoire de biologie clinique,institut de nutrition de tunis,tunis, Tunisia, inserm,u624 'stress cellulaire',marseille-cedex 9, France, laboratoire des substances bioactives,centre de biotechnologie,technopole borj-cedria,tunis, Tunisia, laboratoire de neurophysiologie fonctionnelle et pathologies,département des sciences biologiques,faculté des sciences de tunis,tunis, Tunisia, laboratoire de neurophysiologie fonctionnelle et pathologies,département des sciences biologiques,faculté des sciences de tunis,tunis, Tunisia, laboratoire des substances bioactives,centre de biotechnologie,technopole borj-cedria,tunis, Tunisia
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Authors
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