Dietary supplementation with dried chicory root triggers changes in the blood serum proteins engaged in the clotting process and the innate immune response in growing pigs

The aim of the study was to characterize the systemic immune and metabolic alterations in the blood serum of growing pigs in response to a dietary supplementation with 4% of dried chicory roots. this was achieved by examining the influence of the experimental diet on serum protein changes especially these related with immunology and lipid metabolism. serum proteins with the isoelectric point ranging from ph 3.0 to 10.0 were separated using high resolution two-dimensional electrophoresis. as a result,we found that experimental diet triggered significant changes in 37 protein spots. of these,14 were up-regulated,whereas 23 showed down-regulation. of 37 significantly altered protein spots,24 were successfully identified,representing 14 distinct gene products. implementation of the dried chicory roots into the diet of growing pigs caused a significant down-regulation of apolipoprotein c-ii complement component c6,c-reactive protein,cd14 antigen,c4b binding protein α and β chains,and fibrinogen. piglets fed experimental diet had similar iga,igg and igm concentrations,although the level of igm tended to be lower compared to the control group. it is concluded that diet supplemented with 4% of dried chicory root may exert anti-inflammatory properties and affect lipid metabolism in growing pigs. © 2015,polish physiological society. all rights reserved.