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Transcription factor GATA3 expression is induced by GLS2 overexpression in a glioblastoma cell line but is GLS2-independent in patient-derived glioblastoma
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نویسنده
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majewska e. ,rola r. ,barczewska m. ,marquez j. ,albrecht j. ,szeliga m.
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منبع
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journal of physiology and pharmacology - 2017 - دوره : 68 - شماره : 2 - صفحه:209 -214
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چکیده
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Phosphate-activated glutaminase (ga),a ubiquitous glutamine-metabolizing enzyme,is encoded by two genes,gls and gls2. in mammalian cancers,gls isoforms are perceived as molecules promoting cell proliferation and invasion,whereas the role of gls2 isoforms seems to be more complex and cell type-specific. previous studies have shown abundance of gls and lack of gls2 transcripts in t98g human glioblastoma (gbm) cell line and patient-derived gbm. transfection with gab sequence,the whole gls2 cdna transcript,suppressed malignant phenotype of t98g cells. microarray analysis revealed upregulation of gata3,the product of which has been implicated in suppressing growth of some peripheral cancers. in this study we confirmed a significant upregulation of gata3 expression in the transfected cells both at mrna and protein level. considerable expression of gata3 was also observed in gbm tissues (previously shown as not expressing gls2),while only traces or no gata3 was detected in (gls2-expressing) non-tumorigenic brain samples. in conclusion,while mechanistic relation between gab and gata3 expression is evident following in vitro manipulation of gbm cell line,it does not appear to be an intrinsic property of gbm nor non-tumorigenic brain tissue. © 2017,polish physiological society. all rights reserved.
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کلیدواژه
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GATA3; Glioblastoma tissue; GLS2 gene; Glutaminase isoforms; Human glioblastoma cell line; Non-tumorigenic brain tissue; Transcription factor
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آدرس
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department of neurotoxicology,mossakowski medical research centre,polish academy of sciences,warsaw, Poland, department of neurosurgery and paediatric neurosurgery,medical university of lublin,lublin, Poland, department of neurosurgery,faculty of medical sciences,university of warmia and mazury,olsztyn, Poland, department of molecular biology and biochemistry,faculty of sciences,university of malaga,campus de teatinos,malaga, Spain, department of neurotoxicology,mossakowski medical research centre,polish academy of sciences,warsaw, Poland, department of neurotoxicology,mossakowski medical research centre,polish academy of sciences,warsaw, Poland
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Authors
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