Transforming growth factor-β1 and tumor necrosis factor-α concentration in serum during disturbed lymph flow from a liver in rats

In the last decades an increasing attention has been devoted to the role of lymphatic system in pathomechanism. the disturbed lymph flow from a liver contributes to liver fibrogenesis and probably to hepatocirrhosis. cytokines play a major role in the development of hepatic fibrosis,the wound-healing response of the liver to chronic injury. cytokines in hepatic fibrogenesis may be pro- or antifibrogenic. transforming growth factor-β1 (tgf-β1) is pro-fibrogenic cytokine and plays a key role in liver fibrogenesis. interferon-γ (inf−γ) is anti-fibrogenic by downregulating hepatic stellate cell activation. we described the negative correlation between tumor necrosis factor-α (tnf-α) and ifn-γ concentration in serum during disturbed lymph flow from a liver of rats. tnf-α plays a antifibrogenic role in liver fibrogenesis too. male albino wistar rats weighing 250 – 300 grams were selected for the experiment. the animals were kept in stable condition and were fed a standard diet with no fluid restriction. the rats were divided into 3 groups: group b - mechanical insufficiency was obtained by ligation of hepatic trunc,group k - underwent sham operation,group 0 - rats not subjected to any surgery. the animals were sacrificed for experiment in 1,3,7,14,21,28,35,56 and 103 day after operation. during experiment tgf-β1 and tnf-α concentration in serum were assayed. we observed a positive correlation between tgf-β1 and tnf-α concentration in serum. during disturbed lymph flow from the liver tnf-α plays probably a antifibrogenic role in liver fibrogenesis. © 2017,polish physiological society. all rights reserved.