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Chronic orexin-a (Hypocretin-1) treatment of type 2 diabetic rats improves glucose control and beta-cell functions
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نویسنده
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kaczmarek p. ,skrzypski m. ,pruszynska-oszmalek e. ,sassek m. ,kolodziejski p.a. ,billert m. ,szczepankiewicz d. ,wojciechowicz t. ,maechler p. ,nowak k.w. ,strowski m.z.
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منبع
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journal of physiology and pharmacology - 2017 - دوره : 68 - شماره : 5 - صفحه:669 -681
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چکیده
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Orexin regulates food intake and energy expenditure. here,we test the ability of orexin-a (oxa,hypocretin-1) at improving metabolic control in type 2 diabetic animals and elaborate potential mechanisms of action. rats with experimentally induced type 2 diabetes by a combination of streptozotocin injection and high-fat diet feeding were chronically infused with oxa. in vitro experiments were conducted on isolated pancreatic islets,primary adipocytes and insulin secreting ins-1e cells. oxa improved glucose control,enhanced insulin sensitivity and attenuated pancreatic β-cell loss in type 2 diabetic rats. ex vivo,apoptotic death of pancreatic islets isolated from oxa-treated type 2 diabetic animals as well as the impairment of glucose-stimulated insulin secretion were attenuated,as compared to islets derived from vehicle-treated rats. oxa reduced plasma tumor necrosis factor-α (tnf-α) and non-esterified fatty acids (nefa) levels in type 2 diabetic rats. oxa decreased palmitate- and tnf-α-induced apoptosis of ins-1e cells. oxa improves glucose control by enhancing insulin sensitivity and protecting β-cells from apoptotic cell death in type 2 diabetic animals. © 2017,polish physiological society. all rights reserved.
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کلیدواژه
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Apoptosis; Diabetes; Glukagon-like peptide; Insulin sentistivity; Islets; Orexin; Proinflammatory cytokines; Proliferation; β-cells
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آدرس
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department of animal physiology and biochemistry,poznan university of life sciences,poznan, Poland, department of animal physiology and biochemistry,poznan university of life sciences,poznan, Poland, department of animal physiology and biochemistry,poznan university of life sciences,poznan, Poland, department of animal physiology and biochemistry,poznan university of life sciences,poznan, Poland, department of animal physiology and biochemistry,poznan university of life sciences,poznan, Poland, department of animal physiology and biochemistry,poznan university of life sciences,poznan, Poland, department of animal physiology and biochemistry,poznan university of life sciences,poznan, Poland, department of animal physiology and biochemistry,poznan university of life sciences,poznan, Poland, department of cell physiology and metabolism,university of geneva medical center,geneva, Switzerland, department of animal physiology and biochemistry,poznan university of life sciences,poznan, Poland, department of hepatology and gastroenterology and the interdisciplinary centre of metabolism: endocrinology,diabetes and metabolism,charite-university medicine berlin,berlin,germany,park-klinik weissensee,internal medicine - gastroenterology,berlin, Germany
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Authors
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