Simple impeller systems for maintenance of oil palm culture aggregates

Scaling up of liquid culture systems generally involves moving from the use of simple shake flasks to bioreactors or specialised vessels; this is costly. a new innovation called the two-in-one mpob simple impeller (2-in-1 moslim) was developed using commonly available schott bottles in the laboratory. this system provided simultaneous aeration and agitation (two-inone) in a single device for tissue propagation in liquid culture. the 2-in-1 moslim produced cell aggregates with fresh weight increments of two-to six-fold over 30-40 days. this system was a convenient alternative compared to the conventional shake flask system. multiplication of cultures in the 2-in-1 moslim did not require any shaker or a big space area. this system with a working volume of 300-700 ml used a simple impeller and a pump for agitation and aeration purposes. however,with the 2-in-1 moslim,media replenishment remained a tedious task. to overcome this,modifications were made to the system to enable media replenishment on-site without the need of a sterile hood. the adaptation of 2-in-1 moslim with an earlier innovation,fast transfer technique (mofatt) in liquid culture system,resulted in the development of the simple impeller with fast transfer technique (slim-fatt) system. this new system can be applied to the liquid culture system of any crop with a potential towards automation.