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   18-kDa translocator protein ligand 18 F-FEMPA: Biodistribution and uptake into atherosclerotic plaques in mice  
   
نویسنده Hellberg Sanna ,Silvola Johanna M. U. ,Kiugel Max ,Liljenbäck Heidi ,Savisto Nina ,Li Xiang-Guo ,Thiele Andrea ,Lehmann Lutz ,Heinrich Tobias ,Vollmer Sonja ,Hakovirta Harri ,Laine V. Jukka O. ,Ylä-Herttuala Seppo ,Knuuti Juhani ,Roivainen Anne ,Saraste Antti
منبع journal of nuclear cardiology - 2017 - دوره : 24 - شماره : 3 - صفحه:862 -871
چکیده    Radioligands of 18-kda translocator protein (tspo) expressed on activated macrophages are a potential approach for imaging of inflammation in atherosclerosis. we evaluated a novel tspo-targeted tracer 18f-fempa for the detection of atherosclerotic plaque inflammation in mice. the distribution kinetics of 18f-fempa was evaluated by in vivo pet/ct imaging. 18f-fempa uptake was compared in atherosclerotic (ldlr−/−apob100/100, n = 10) and healthy mice (c57bl/6 n, n = 7) ex vivo at twenty minutes post-injection. biodistribution was analyzed from harvested tissue samples, and aortas were sectioned for autoradiography. aortas of ldlr−/−apob100/100 mice showed large, macrophage-rich atherosclerotic plaques. in vivo, 18f-fempa showed rapid blood clearance but no difference in aortic uptake between atherosclerotic and healthy mice. in the mice studied ex vivo at 20 minutes post-injection, quantification of radioactivity in the whole aorta showed 1.3-fold higher 18f-fempa accumulation in atherosclerotic than healthy mice (p = .028). autoradiography showed higher tracer uptake in plaque areas with high macrophage content as compared with areas of no macrophages (count densities 190 ± 54 vs 40 ± 13 psl/mm2, p < .001), but the uptake in the plaques was not higher than in the normal vessel wall (230 ± 78 psl/mm2). in vitro blocking showed specific accumulation in mouse and human atherosclerotic plaques. immunohistochemistry confirmed co-localization of tspo and macrophages. 18f-fempa shows rapid blood clearance and uptake in the mouse aorta. uptake in atherosclerotic plaques correlated with the amount of macrophages, but did not exceed that in the normal vessel wall.
کلیدواژه Atherosclerosis ,18F-FEMPA ,inflammation ,PET/CT imaging ,18-kDa translocator protein
آدرس University of Turku, Finland, University of Turku, Finland, University of Turku, Finland, University of Turku, Finland, University of Turku, Finland, University of Turku, Finland, Bayer Pharma AG, Germany, Bayer Pharma AG, Germany, Bayer Pharma AG, Germany, Bayer Pharma AG, Germany, University of Turku, Department of Surgery, Finland, Turku University Hospital, Department of Pathology, Finland, University of Eastern Finland, Finland, University of Turku, Finland. Turku University Hospital, Finland, University of Turku, Finland. Turku University Hospital, Finland, University of Turku, Finland. Turku University Hospital, Finland. Turku University Hospital and University of Turku, Finland
 
     
   
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