Radiation induced TLR-8 expression and TGF-β/TNF-α production in BV 2 microglial cells [BV 2 mikroglial hücrelerde radyasyona baǧli{dotless} TGF-β/TNF-α yapi{dotless}mi{dotless} ve TLR-8 arti{dotless}şi{dotless}]

Objective: to observe the radiation-induced tlr-8 mrna expression and production of proinflammatory cytokines in bv2 microglial cells. methods: tlr-8 mrna expression and distribution in bv-2 cells was analyzed by fluorescent quantitative pcr and flow measurement at different time point (1,3,6,12,24,48 and 72 h) after a single 16 gy dose of radiation,or at 24 h after various doses of radiation (2,5,10,16,20,30 and 40 gy). elisa was used to determinate the concentration of tnf-α and tgf-β in the supernatant of bv-2 cells. results: the expression of tlr-8 mrna in bv2 microglia cells was 0.000639 ± 0.00016,with 41.98% in the membrane and 32.08% in the cell. after 16 gy radiation,the tlr-8 mrna expression increased at 1h after radiation,with peak at 24 h. tlr-8 mrna increased after 2 gy radiation,and the value increased to top at 16 gy. there was a steady increase in the concentration of tgf-β in the supernatant of bv 2 microglial cells 48 h later. a slight effect on tnf-α was also found. conclusion: the data suggests that tlr-8-induced activation of microglia and the release of tgf-β might be responsible for brain injury induced by radiation.