Rat bone marrow stromal cells derived neural stem cell-like cells could differentiate into functional schwann cell-like cells in vitro [Si{dotless}çan kemik i̇liǧi stromal hücrelerden türetilmiş nöral kök hücre benzeri hücreler i̇n vitro i̇şlev görebilen schwann benzeri hücrelere deǧişebilirler]

Objective: to induce neural stem cell (nsc)-like cells derived from rat bone marrow stromal cells (bmscs) into functional schwann cell (sc)-like cells. methods: in this study,rat bmscs were cultured,identified and converted into nsc-like cells. nsc-like cells were then induced into sc-like cells. the phenotype and functional characteristics of sc-like cells were identified carefully. results: rat bmscs could be converted into nsc-like cells. the nsc-like cells could differentiate not only into neuron-like cells,but also into functional sc-like cells. sc-like cells were bi-or tri-polar in shape and were positive for sc markers p75,gfap and s-100. besides these,sc-like cells could express myelin protein p0 when intracellular camp was elevated. sc-like cells could also form myelin structures with pc12 cell neurites in vitro. conclusions: these findings indicated that bmscs-derived nsc-like cells could differentiate into functional sc-like cells,which may be useful for the treatment of demyelinating diseases of nervous system.