Comparative effects of antioxidants on chronic ethanol-induced oxidative stress in rat hippocampus [Si{dotless}çan hipokampusunda kronik etanolün oluşturduǧu oksidatif stres üzerine antioksidanlari{dotless}n karşi{dotless}laşti{dotless}rmali{dotless} etkileri]

Objective: long-term ethanol exposure can cause serious damages on central nervous system functions. the aim of the study was to investigate the effects of several antioxidant agents on chronic ethanol-induced oxidative stress in rat brain. material and methods: oxidative stress was evaluated by measuring malondialdehyde (mda),superoxide dismutase (sod) and glutathione peroxidase (gsh-px),levels in the brain tissue. ethanol was given to adult male wistar rats by a liquid diet for 28 days. control rats were fed by no ethanol contained izocaloric liquid diet. melatonin (4 mg),ebselen (20 mg),proantocyanidin (pac) (100 mg),vitamin e (100 mg) and vitamin c (100 mg) were applied to rats by oral route with gavages for 7 days from 22 ndday of ethanol administration. blood alcohol levels were measured by gas chromatography technique. mda,sod and gsh-px levels were measured in hippocampal tissue. results: while chronic exposure to ethanol caused a significant increase in mda level,it significantly decreased both sod and gsh-px levels in hippocampal tissue. all antioxidants used in the study significantly reversed both the decrease in sod and the increase in mda levels. vitamin e and vitamin c were ineffective on decreased gsh-px level in ethanol administered rats. conclusions: our results suggest that chronic ethanol exposition causes oxidative stress responses that can be reversed by melatonin,ebselen,pac,vitamin e and vitamin c treatments in hippocampal area of rat brain. in addition,melatonin,ebselen and pac were more effective than vitamin e and vitamin c.