The effects of female sexual hormones and estrogen receptor agonists on the NF-κB p50 and p65 levels following TBI in rat intestine [Si{dotless}çan Barsaǧi{dotless}nda Travmatik Beyin Yaralanmasi{dotless} sonrasi{dotless} Dişi Seksüel Hormonlari{dotless}n ve Östrogen Reseptör Agonistlerin NF-κB p50 ve p65 Seviyelerine Etkisi]

Objectives: the purpose of this study was to evaluate the role of female sexual hormones on the intestinal nf-κb levels following traumatic brain injury (tbi) induction. materials and methods: diffuse tbi was induced by marmarou method in ovariectomized (ovx) female rats. rats randomly assigned into 10 groups: intact,sham+ovx,tbi,tbi+ovx,vehicle,estradiol (e2),progesterone (p),e2+p,ppt and dpn (estrogen receptor alpha and beta agonists,respectively). the intestinal nf-κb p50 and p65 levels were measured by elisa method 5 days after tbi. results: the intestinal levels of nf-κb p65 were decreased in tbi group vs intact group (p<0.001). all treatment groups had caused a significant reduction in intestinal levels of nf- κb p65 (p<0.001) vs ovx + tbi. the trauma induction doesn't have any effect on the intestinal nf-κb p50 levels. the ovx+tbi animals treated with estrogen,progesterone and estrogen+ progesterone showed a significant reduction (p<0.05) vs ovx + tbi (p<0.001). ppt had caused a significant reduction in intestinal levels of nf-κb p50 (p <0.05) compared to ovx + tbi. ppt and dpn significantly increased the intestinal levels of nf-κb p50 vs vehicle group (p <0.05). conclusion: the binding activity of different subunits of nf-κb (p50 and p65) varies following traumatic brain injury. also,because the nf-κb binding activity reduced in intestinal tissue after treatment with steroids,it is possible that steroids do their performance by reducing the activity of nf-κb and thereby reduce the production of intestinal inflammatory cytokines.