Characterization of photothrombotic cerebral infarction model at sensorimotor area of functional map in rat [Si{dotless}çanda fonksiyonel haritani{dotless}n sensorimotor alani{dotless}nda fototrombotik serebral İnfarkti{dotless}n karakterizasyonu]

Purpose: the purpose of this study is to induce a photothrombotic cerebral infarction (ptci) model with sensorimotor dysfunction in rats with a cortical functional map and to find the time course of neurological and morphological changes. methods: using sixteen rats,a ptci was produced by using an intravenous injection of rose bengal dye (20mg/kg) and exposed to cold light for 20 min at a position of 1 mm anterior to bregma and 3.5 mm right lateral of midline,with the help of a cortical functional map. serial changes were evaluated in magnetic resonance images,in neurology by rota-rod test (rrt) and dynamic plantar aesthesiometer (dpa),and in pathology over time from day 1 through 14. results: the brain infarction was successfully induced in all 16 rats without a single death. infarction volume was reduced gradually over time from 147 mm3 to 100,47,35,and 27 mm3 on days 1,3,7,11,and 14 respectively. by rrt evaluation,significant increased neurological dysfunction from 41% to 49% was observed in 2 weeks. a dpa examination revealed a dysfunction of contralateral forelimb from 181% to 163% within 2 weeks. hemorrhage and liquefaction were observed by mri and histopathology. conclusions: sensorimotor dysfunction continued for 2 weeks in the ptci model by targeting relevant cortex areas in the rat brain guided by a cortical functional map.