The relationship between melatonin and cannabinoid-1 receptor in cortical dysplasia generated rats [Kortikal displazi oluşturulmuş si{dotless}çanlarda melatonin ile kannabinoid reseptörü i̇lişkisi]

Objectives: cortical dysplasia is a developmental malformation that occurs as a result of defects in neuronal proliferation,migration and organization during the formation of brain cortex layers. cannabinoid-1 receptor (cb1r) plays a role in brain cortex development. melatonin neuroprotective is an agent. in this study using an experimental cortical dysplasia model,cb1r expression and the effect of melatonin on cb1r were investigated. methods: eighteen female wistar albino rats were randomly divided into three groups (n=6). a single dose of intraperitoneal (i.p) saline (20 ml/kg) was administered in the 15th day of pregnancy in the rats group 1 (sham group). a single dose of i.p bcnu (20 mg/kg) was administered in the 15th day of pregnancy in the rats in group 2 (bcnu) and group 3 (bcnu+ melatonin). in addition,i.p melatonin (10 mg/kg) starting on day 15 of pregnancy and continuing until the day of delivery were administered to the rats in group 3 (bcnu+melatonin). newborn brain sections were investigated using luxol fast blue and anti-cb1r staining. results: while the sham group had cerebral cortex stratification compatible with the normal newborn histology,it was observed that the stratification was disrupted in the group 2 and group 3. in the observed brain regions (cerebral cortex,cerebellar cortex and hippocampus),it was observed that cb1r immunoreactivity decreased in group 2 and 3. conclusion: in an experimental cortical dysplasia model,it was seen that the administration of melatonin revealed a protective effect against the decrease in cb1r expression. it has been suggested that a decrease in cb1r expression would also play a role in cortical dysplasia formation mechanisms.