Protective effects of vitamin D3 on anxiety-like behavior and the total antioxidant power following the local injection of lysophosphatidylcholine in the adult rat dorsal hippocampus [Erişkin sıçan dorsal hippokampusunde lokal lysophosphatidylcholine enjeksiyonunu takiben D3 vitamininin anksiyete benzeri davranışlar üzerine etkisi ve total antioksidan gücü]

Background: studies have reported levels of anxiety in multiple sclerosis (ms) patients. considering the involvement of hippocampus in anxiety,an attempt is made in this study to investigate the effects of vitamin d3 on anxiety-related behavior and total antioxidant power in the dorsal hippocampus (ca1 area) following the induction of demyelination by lysophosphatidylcholine (lpc). methods: animals were divided into four groups; each group contained eight animals. control group: animals received 2 μl saline in ca1 area; sham group: animals received 150 μl sesame oil intraperitoneally; lpc group: animals received lpc by stereotaxic intrahippocampal injection of 2μl lpc in ca1 area; vitamin d3- treated group: animals were treated with vitamin d3 at doses of 5μg/kg intraperitoneally for 7,14 and 21 days after receiving lpc. elevated plus maze (epm) was used for testing the anxiety. the ferric reducing ability of plasma (frap) assay is chosen to assess the free radical scavenging power of vitamin d3. results: bilateral injection of lpc caused demyelination and induced an anxiogenic-like effect which led to a reduction in the open arm time percentage (oat%) and open arm entries percentage (oae%). vitamin d3 administration increased the oat% and oae% without effects on locomotor activity compared to lpc-treated rats. also,our results indicate that the vitamin d3 administration caused an increase in the total antioxidant power. conclusion: it can be concluded that the vitamin d3 administration can produce clear anxiolytic effects through its positive effects on the total antioxidant power. © 2015,ege university press. all rights reserved.