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Delayed inflammatory mRNA and protein expression after spinal cord injury
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نویسنده
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byrnes k.r. ,washington p.m. ,knoblach s.m. ,hoffman e. ,faden a.i.
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منبع
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journal of neuroinflammation - 2011 - دوره : 8 - شماره : 0
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چکیده
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Background: spinal cord injury (sci) induces secondary tissue damage that is associated with inflammation. we have previously demonstrated that inflammation-related gene expression after sci occurs in two waves - an initial cluster that is acutely and transiently up-regulated within 24 hours,and a more delayed cluster that peaks between 72 hours and 7 days. here we extend the microarray analysis of these gene clusters up to 6 months post-sci.methods: adult male rats were subjected to mild,moderate or severe spinal cord contusion injury at t9 using a well-characterized weight-drop model. tissue from the lesion epicenter was obtained 4 hours,24 hours,7 days,28 days,3 months or 6 months post-injury and processed for microarray analysis and protein expression.results: anchor gene analysis using c1qb revealed a cluster of genes that showed elevated expression through 6 months post-injury,including galectin-3,p22phox,gp91phox,cd53 and progranulin. the expression of these genes occurred primarily in microglia/macrophage cells and was confirmed at the protein level using both immunohistochemistry and western blotting. as p22phoxand gp91phoxare components of the nadph oxidase enzyme,enzymatic activity and its role in sci were assessed and nadph oxidase activity was found to be significantly up-regulated through 6 months post-injury. further,treating rats with the nonspecific,irreversible nadph oxidase inhibitor diphenylene iodinium (dpi) reduced both lesion volume and expression of chronic gene cluster proteins one month after trauma.conclusions: these data demonstrate that inflammation-related genes are chronically up-regulated after sci and may contribute to further tissue loss. © 2011 byrnes et al; licensee biomed central ltd.
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کلیدواژه
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Chronic; Dpi; Galectin-3; Inflammation; Mac-2 ,; Microarray; Microglia; Nadph oxidase
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آدرس
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department of neuroscience,georgetown university medical center,reservoir rd,nw,washington,dc (20057),united states,department of anatomy,physiology and genetics,uniformed services university of the health sciences,jones bridge road,bethesda,md (20814), United States, department of neuroscience,georgetown university medical center,reservoir rd,nw,washington,dc (20057), United States, center for genetic medicine,children's national medical center,michigan ave,nw,washington,dc (20010),united states,department of integrative systems biology,george washington university school of medicine and health sciences,i street nw,washington,dc (20037), United States, center for genetic medicine,children's national medical center,michigan ave,nw,washington,dc (20010),united states,department of integrative systems biology,george washington university school of medicine and health sciences,i street nw,washington,dc (20037), United States, department of neuroscience,georgetown university medical center,reservoir rd,nw,washington,dc (20057),united states,department of anesthesiology,university of maryland school of medicine,s. greene st,baltimore,md (21201),united states,center for shock,trauma and anesthesiology research (star),university of maryland school of medicine,s. greene st,baltimore,md (21201), United States
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Authors
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