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   Gliovascular and cytokine interactions modulate brain endothelial barrier in vitro  
   
نویسنده chaitanya g.v. ,cromer w.e. ,wells s.r. ,jennings m.h. ,couraud p.o. ,romero i.a. ,weksler b. ,erdreich-epstein a. ,mathis j.m. ,minagar a. ,alexander j.s.
منبع journal of neuroinflammation - 2011 - دوره : 8 - شماره : 0
چکیده    The glio-vascular unit (g-unit) plays a prominent role in maintaining homeostasis of the blood-brain barrier (bbb) and disturbances in cells forming this unit may seriously dysregulate bbb. the direct and indirect effects of cytokines on cellular components of the bbb are not yet unclear. the present study compares the effects of cytokines and cytokine-treated astrocytes on brain endothelial barrier. 3-dimensional transwell co-cultures of brain endothelium and related-barrier forming cells with astrocytes were used to investigate gliovascular barrier responses to cytokines during pathological stresses. gliovascular barrier was measured using trans-endothelial electrical resistance (teer),a sensitive index of in vitro barrier integrity. we found that neither tnf-α,il-1β or ifn-γ directly reduced barrier in human or mouse brain endothelial cells or ecv-304 barrier (independent of cell viability/metabolism),but found that astrocyte exposure to cytokines in co-culture significantly reduced endothelial (and ecv-304) barrier. these results indicate that the barrier established by human and mouse brain endothelial cells (and other cells) may respond positively to cytokines alone,but that during pathological conditions,cytokines dysregulate the barrier forming cells indirectly through astrocyte activation involving reorganization of junctions,matrix,focal adhesion or release of barrier modulating factors (e.g. oxidants,mmps). © 2011 chaitanya et al; licensee biomed central ltd.
کلیدواژه Astrocytes; Brain endothelium; Co-culture; Ifn-γ; Il-1β; Mono-culture; Tnf-α
آدرس department of molecular and cellular physiology,school of graduate studies,louisiana state university health sciences center-shreveport,1501 kings hwy,shreveport,la 71130, United States, cell biology and anatomy,school of graduate studies,louisiana state university health sciences center-shreveport,1501 kings hwy,shreveport,la 71130, United States, department of molecular and cellular physiology,school of graduate studies,louisiana state university health sciences center-shreveport,1501 kings hwy,shreveport,la 71130, United States, department of molecular and cellular physiology,school of graduate studies,louisiana state university health sciences center-shreveport,1501 kings hwy,shreveport,la 71130, United States, inserm,u1016,institut cochin,paris,france,cnrs,umr8104,paris,france,univ paris descartes,paris, France, department of biological sciences,the open university,milton keynes, United Kingdom, department of biological sciences,the open university,milton keynes, United Kingdom, division of hematology-oncology,departments of pediatrics and pathology,the saban research institute at children's hospital los angeles and keck school of medicine,university of southern california,4650 sunset boulevard,los angeles,ca 90027, United States, cell biology and anatomy,school of graduate studies,louisiana state university health sciences center-shreveport,1501 kings hwy,shreveport,la 71130, United States, department of neurology,school of medicine,louisiana state university health sciences center-shreveport,1501 kings hwy,shreveport,la 71130, United States, department of molecular and cellular physiology,school of graduate studies,louisiana state university health sciences center-shreveport,1501 kings hwy,shreveport,la 71130,united states,department of medicine,weill medical college,1300 york ave,new york,ny-10065, United States
 
     
   
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