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Mesenchymal stem cells inhibit lipopolysaccharide-induced inflammatory responses of BV2 microglial cells through TSG-6
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نویسنده
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liu y. ,zhang r. ,yan k. ,chen f. ,huang w. ,lv b. ,sun c. ,xu l. ,li f. ,jiang x.
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منبع
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journal of neuroinflammation - 2014 - دوره : 11 - شماره : 0
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چکیده
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Microglia are the primary immunocompetent cells in brain tissue and microglia-mediated inflammation is associated with the pathogenesis of various neuronal disorders. recently,many studies have shown that mesenchymal stem cells (mscs) display a remarkable ability to modulate inflammatory and immune responses through the release of a variety of bioactive molecules,thereby protecting the central nervous system. previously,we reported that mscs have the ability to modulate inflammatory responses in a traumatic brain injury model and that the potential mechanisms may be partially attributed to upregulated tnf-α stimulated gene/protein 6 (tsg-6) expression. however,whether tsg-6 exerts an anti-inflammatory effect by affecting microglia is not fully understood. in this study,we investigated the anti-inflammatory effects of mscs and tsg-6 in an in vitro lipopolysaccharide (lps)-induced bv2 microglial activation model. we found that mscs and tsg-6 significantly inhibited the expression of pro-inflammatory mediators in activated microglia. however,msc effects on microglia were attenuated when tsg-6 expression was silenced. in addition,we found that the activation of nuclear factor (nf)-κb and mitogen-activated protein kinase (mapk) pathways in lps-stimulated bv2 microglial cells was significantly inhibited by tsg-6. furthermore,we found that the presence of cd44 in bv2 microglial cells was essential for msc- and tsg-6-mediated inhibition of pro-inflammatory gene expression and of nf-κb and mapk activation in bv2 microglial cells. the results of this study suggest that mscs can modulate microglia activation through tsg-6 and that tsg-6 attenuates the inflammatory cascade in activated microglia. our study indicates that novel mechanisms are responsible for the immunomodulatory effect of mscs on microglia and that mscs,as well as tsg-6,might be promising therapeutic agents for the treatment of neurotraumatic injuries or neuroinflammatory diseases associated with microglial activation. © 2014 liu et al.
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آدرس
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national key clinic specialty,the neurosurgery institute of guangdong province,guangdong provincial key laboratory on brain function repair and regeneration,department of neurosurgery,zhujiang hospital,southern medical university,253# gongye road,guangzhou,510282, China, national key clinic specialty,the neurosurgery institute of guangdong province,guangdong provincial key laboratory on brain function repair and regeneration,department of neurosurgery,zhujiang hospital,southern medical university,253# gongye road,guangzhou,510282, China, national key clinic specialty,the neurosurgery institute of guangdong province,guangdong provincial key laboratory on brain function repair and regeneration,department of neurosurgery,zhujiang hospital,southern medical university,253# gongye road,guangzhou,510282, China, national key clinic specialty,the neurosurgery institute of guangdong province,guangdong provincial key laboratory on brain function repair and regeneration,department of neurosurgery,zhujiang hospital,southern medical university,253# gongye road,guangzhou,510282, China, national key clinic specialty,the neurosurgery institute of guangdong province,guangdong provincial key laboratory on brain function repair and regeneration,department of neurosurgery,zhujiang hospital,southern medical university,253# gongye road,guangzhou,510282, China, national key clinic specialty,the neurosurgery institute of guangdong province,guangdong provincial key laboratory on brain function repair and regeneration,department of neurosurgery,zhujiang hospital,southern medical university,253# gongye road,guangzhou,510282, China, national key clinic specialty,the neurosurgery institute of guangdong province,guangdong provincial key laboratory on brain function repair and regeneration,department of neurosurgery,zhujiang hospital,southern medical university,253# gongye road,guangzhou,510282, China, national key clinic specialty,the neurosurgery institute of guangdong province,guangdong provincial key laboratory on brain function repair and regeneration,department of neurosurgery,zhujiang hospital,southern medical university,253# gongye road,guangzhou,510282, China, national key clinic specialty,the neurosurgery institute of guangdong province,guangdong provincial key laboratory on brain function repair and regeneration,department of neurosurgery,zhujiang hospital,southern medical university,253# gongye road,guangzhou,510282, China, national key clinic specialty,the neurosurgery institute of guangdong province,guangdong provincial key laboratory on brain function repair and regeneration,department of neurosurgery,zhujiang hospital,southern medical university,253# gongye road,guangzhou,510282, China
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Authors
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