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Involvement of phosphatase and tensin homolog deleted from chromosome 10 in rodent model of neuropathic pain
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نویسنده
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huang s.-y. ,sung c.-s. ,chen w.-f. ,chen c.-h. ,feng c.-w. ,yang s.-n. ,hung h.-c. ,chen n.-f. ,lin p.-r. ,chen s.-c. ,wang h.-m.d. ,chu t.-h. ,tai m.-h. ,wen z.-h.
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منبع
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journal of neuroinflammation - 2015 - دوره : 12 - شماره : 1
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چکیده
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Background: many cancer research studies have extensively examined the phosphatase and tensin homolog deleted from chromosome 10 (pten) pathway. there are only few reports that suggest that pten might affect pain; however,there is still a lack of evidence to show the role of pten for modulating pain. here,we report a role for pten in a rodent model of neuropathic pain. results: we found that chronic constriction injury (cci) surgery in rats could elicit downregulation of spinal pten as well as upregulation of phosphorylated pten (phospho-pten) and phosphorylated mammalian target of rapamycin (phospho-mtor). after examining such changes in endogenous pten in neuropathic rats,we explored the effects of modulating the spinal pten pathway on nociceptive behaviors. the normal rats exhibited mechanical allodynia after intrathecal (i.t.) injection of adenovirus-mediated pten antisense oligonucleotide (ad-antisense pten). these data indicate the importance of downregulation of spinal pten for nociception. moreover,upregulation of spinal pten by i.t. adenovirus-mediated pten (ad-pten) significantly prevented cci-induced development of nociceptive sensitization,thermal hyperalgesia,mechanical allodynia,cold allodynia,and weight-bearing deficits in neuropathic rats. furthermore,upregulation of spinal pten by i.t. ad-pten significantly attenuated cci-induced microglia and astrocyte activation,upregulation of tumor necrosis factor-α (tnf-α) and phospho-mtor,and downregulation of pten in neuropathic rats 14 days post injury. conclusions: these findings demonstrate that pten plays a key,beneficial role in a rodent model of neuropathic pain. © huang et al.
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کلیدواژه
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Astrocyte; Chronic constriction injury; Intrathecal; Neuroinflammation
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آدرس
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national sun yat-sen university,department of marine biotechnology and resources,no. 70,lienhai road,kaohsiung,80424,taiwan,center for neuroscience,national sun yat-sen university,no. 70,lienhai road,kaohsiung,80424, Taiwan, taipei veterans general hospital,department of anesthesiology,no. 201,section 2,shipai road,taipei,11217,taiwan,school of medicine,national yang-ming university,no. 155,section 2,linong street,taipei,11221, Taiwan, national sun yat-sen university,department of marine biotechnology and resources,no. 70,lienhai road,kaohsiung,80424,taiwan,kaohsiung chang gung memorial hospital and chang gung university college of medicine,department of neurosurgery,no. 123,dapi road,kaohsiung,83301,taiwan,xiamen chang gung memorial hospital,department of neurosurgery,no. 123,xiafei road,fujian,361026, China, national sun yat-sen university,department of marine biotechnology and resources,no. 70,lienhai road,kaohsiung,80424,taiwan,doctoral degree program in marine biotechnology,national sun yat-sen university and academia sinica,no. 70,lienhai road,kaohsiung,80424, Taiwan, national sun yat-sen university,department of marine biotechnology and resources,no. 70,lienhai road,kaohsiung,80424,taiwan,doctoral degree program in marine biotechnology,national sun yat-sen university and academia sinica,no. 70,lienhai road,kaohsiung,80424, Taiwan, e-da hospital,i-shou university,school of medicine,college of medicine and department of pediatrics,no. 1,yida road,kaohsiung,82445, Taiwan, national sun yat-sen university,department of marine biotechnology and resources,no. 70,lienhai road,kaohsiung,80424,taiwan,doctoral degree program in marine biotechnology,national sun yat-sen university and academia sinica,no. 70,lienhai road,kaohsiung,80424, Taiwan, national sun yat-sen university,department of marine biotechnology and resources,no. 70,lienhai road,kaohsiung,80424,taiwan,kaohsiung armed forces general hospital,division of neurosurgery,department of surgery,no. 2,zhongzheng 1st road,kaohsiung,80284, Taiwan, institute of biomedical sciences,national sun yat-sen university,#70 lienhai road,kaohsiung,80424, Taiwan, center for neuroscience,national sun yat-sen university,no. 70,lienhai road,kaohsiung,80424,taiwan,institute of biomedical sciences,national sun yat-sen university,#70 lienhai road,kaohsiung,80424, Taiwan, national sun yat-sen university,department of marine biotechnology and resources,no. 70,lienhai road,kaohsiung,80424,taiwan,kaohsiung medical university,department of fragrance and cosmetic science,no. 100,shiquan 1st road,kaohsiung,80708,taiwan,graduate institute of natural products,kaohsiung medical university,no. 100,shiquan 1st road,kaohsiung,80708,taiwan,center for stem cell research,kaohsiung medical university,no. 100,shiquan 1st road,kaohsiung,80708, Taiwan, institute of biomedical sciences,national sun yat-sen university,#70 lienhai road,kaohsiung,80424, Taiwan, center for neuroscience,national sun yat-sen university,no. 70,lienhai road,kaohsiung,80424,taiwan,institute of biomedical sciences,national sun yat-sen university,#70 lienhai road,kaohsiung,80424,taiwan,national sun yat-sen university,department of biological sciences,no. 70,lienhai road,kaohsiung,80424, Taiwan, national sun yat-sen university,department of marine biotechnology and resources,no. 70,lienhai road,kaohsiung,80424,taiwan,doctoral degree program in marine biotechnology,national sun yat-sen university and academia sinica,no. 70,lienhai road,kaohsiung,80424,taiwan,national sun yat-sen university,marine biomedical laboratory and center for translational biopharmaceuticals,department of marine biotechnology and resources,no. 70,lienhai road,kaohsiung,80424, Taiwan
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Authors
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