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Epistasis analysis links immune cascades and cerebral amyloidosis
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نویسنده
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benedet a.l. ,labbe a. ,lemay p. ,zimmer e.r. ,pascoal t.a. ,leuzy a. ,mathotaarachchi s. ,mohades s. ,shin m. ,dionne-laporte a. ,beaudry t. ,picard c. ,gauthier s. ,poirier j. ,rouleau g. ,rosa-neto p.
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منبع
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journal of neuroinflammation - 2015 - دوره : 12 - شماره : 1
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چکیده
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Background: several lines of evidence suggest the involvement of neuroinflammatory changes in alzheimer's disease (ad) pathophysiology such as amyloidosis and neurodegeneration. in fact,genome-wide association studies (gwas) have shown a link between genes involved in neuroinflammation and ad. in order to further investigate whether interactions between candidate genetic variances coding for neuroinflammatory molecules are associated with brain amyloid β (aβ) fibrillary accumulation,we conducted an epistasis analysis on a pool of genes associated with molecular mediators of inflammation. methods: [18f]florbetapir positron emission tomography (pet) imaging was employed to assess brain aβ levels in 417 participants from adni-go/2 and posteriorly 174 from adni-1. il-1β,il4,il6,il6r,il10,il12,il18,c5,and c9 genes were chosen based on previous studies conducted in ad patients. using the [18f]florbetapir standardized uptake value ratio (suvr) as a quantitative measure of fibrillary aβ,epistasis analyses were performed between two sets of markers of immune-related genes using gender,diagnosis,and apolipoprotein e (apoe) as covariates. voxel-based analyses were also conducted. the results were corrected for multiple comparison tests. cerebrospinal fluid (csf) aβ1-42/phosphorylated tau (p-tau) ratio concentrations were used to confirm such associations. results: epistasis analysis unveiled two significant single nucleotide polymorphism (snp)-snp interactions (false discovery rate (fdr) threshold 0.1),both interactions between c9 gene (rs261752) and il6r gene (rs4240872,rs7514452). in a combined sample,the interactions were confirmed (p≥10-5) and associated with amyloid accumulation within cognitively normal and ad spectrum groups. voxel-based analysis corroborated initial findings. csf biomarker (aβ1-42/p-tau) confirmed the genetic interaction. additionally,rs4240872 and rs7514452 snps were shown to be associated with csf and plasma concentrations of il6r protein. conclusions: certain allele combinations involving il6r and c9 genes are associated with aβ burden in the brain. hypothesis-driven search for epistasis is a valuable strategy for investigating imaging endophenotypes in complex neurodegenerative diseases. © 2015 benedet et al.
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آدرس
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mcgill university research centre for studies in aging,translational neuroimaging laboratory,6825 lasalle blvd,montreal,qc h4h 1r3,canada,ministry of education of brazil,capes foundation,brasília, Brazil, mcgill university,douglas hospital research centre,montreal,canada,mcgill university,department of epidemiology,biostatistics and occupational health,montreal,canada,mcgill university,department of psychiatry,montreal, Canada, université de montréal,department of biochemistry,montréal, Canada, mcgill university research centre for studies in aging,translational neuroimaging laboratory,6825 lasalle blvd,montreal,qc h4h 1r3,canada,federal university of rio grande do sul,department of biochemistry,porto alegre,brazil,pontifical catholic university of rio grande do sul (pucrs),brain institute of rio grande do sul (brains),porto alegre, Brazil, mcgill university research centre for studies in aging,translational neuroimaging laboratory,6825 lasalle blvd,montreal,qc h4h 1r3, Canada, mcgill university research centre for studies in aging,translational neuroimaging laboratory,6825 lasalle blvd,montreal,qc h4h 1r3,canada,karolinska institutet,department of nvs,center for alzheimer research,translational alzheimer neurobiology,stockholm,sweden,mcgill university,alzheimer's disease research unit,mcgill university research centre for studies in aging,montreal, Canada, mcgill university research centre for studies in aging,translational neuroimaging laboratory,6825 lasalle blvd,montreal,qc h4h 1r3, Canada, mcgill university research centre for studies in aging,translational neuroimaging laboratory,6825 lasalle blvd,montreal,qc h4h 1r3, Canada, mcgill university research centre for studies in aging,translational neuroimaging laboratory,6825 lasalle blvd,montreal,qc h4h 1r3, Canada, mcgill university,department of neurology and neurosurgery,montreal,canada,montreal neurological institute,montreal, Canada, mcgill university research centre for studies in aging,translational neuroimaging laboratory,6825 lasalle blvd,montreal,qc h4h 1r3, Canada, mcgill university,douglas hospital research centre,montreal, Canada, mcgill university,department of neurology and neurosurgery,montreal, Canada, mcgill university,douglas hospital research centre,montreal,canada,mcgill university,alzheimer's disease research unit,mcgill university research centre for studies in aging,montreal,canada,mcgill university,department of neurology and neurosurgery,montreal, Canada, mcgill university,department of neurology and neurosurgery,montreal,canada,montreal neurological institute,montreal, Canada, mcgill university research centre for studies in aging,translational neuroimaging laboratory,6825 lasalle blvd,montreal,qc h4h 1r3,canada,mcgill university,alzheimer's disease research unit,mcgill university research centre for studies in aging,montreal,canada,mcgill university,department of neurology and neurosurgery,montreal,canada,montreal neurological institute,montreal, Canada
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Authors
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