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γδT cells but not αβT cells contribute to sepsis-induced white matter injury and motor abnormalities in mice
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نویسنده
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zhang x. ,rocha-ferreira e. ,li t. ,vontell r. ,jabin d. ,hua s. ,zhou k. ,nazmi a. ,albertsson a.-m. ,sobotka k. ,ek j. ,thornton c. ,hagberg h. ,mallard c. ,leavenworth j.w. ,zhu c. ,wang x.
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منبع
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journal of neuroinflammation - 2017 - دوره : 14 - شماره : 1
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چکیده
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Background: infection and sepsis are associated with brain white matter injury in preterm infants and the subsequent development of cerebral palsy. methods: in the present study,we used a neonatal mouse sepsis-induced white matter injury model to determine the contribution of different t cell subsets (αβt cells and γδt cells) to white matter injury and consequent behavioral changes. c57bl/6j wild-type (wt),t cell receptor (tcr) δ-deficient (tcrd -/-,lacking γδt cells),and tcrα-deficient (tcra -/-,lacking αβt cells) mice were administered with lipopolysaccharide (lps) at postnatal day (pnd) 2. brain myelination was examined at pnds 12,26,and 60. motor function and anxiety-like behavior were evaluated at pnd 26 or 30 using digigait analysis and an elevated plus maze. results: white matter development was normal in tcrd -/- and tcrα -/- compared to wt mice. lps exposure induced reductions in white matter tissue volume in wt and tcrα -/- mice,but not in the tcrd -/- mice,compared with the saline-treated groups. neither lps administration nor the t cell deficiency affected anxiety behavior in these mice as determined with the elevated plus maze. digigait analysis revealed motor function deficiency after lps-induced sepsis in both wt and tcrα -/- mice,but no such effect was observed in tcrd -/- mice. conclusions: our results suggest that γδt cells but not αβt cells contribute to sepsis-induced white matter injury and subsequent motor function abnormalities in early life. modulating the activity of γδt cells in the early stages of preterm white matter injury might represent a novel therapeutic strategy for the treatment of perinatal brain injury. © 2017 the author(s).
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کلیدواژه
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Behavior tests; Preterm; Sepsis; T lymphocytes; White matter injury
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آدرس
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the third affiliated hospital of zhengzhou university,henan key laboratory of child brain injury,zhengzhou,450052,china,university of gothenburg,perinatal center,department of neuroscience and physiology,sahlgrenska academy,box 432,gothenburg,405 30, Sweden, university of gothenburg,department of obstetrics and gynecology,sahlgrenska academy,gothenburg, Sweden, the third affiliated hospital of zhengzhou university,henan key laboratory of child brain injury,zhengzhou,450052,china,university of gothenburg,center for brain repair and rehabilitation,institute of neuroscience and physiology,sahlgrenska academy,box 436,gothenburg,405 30, Sweden, st. thomas' hospital,department of perinatal imaging and health,division of imaging sciences and biomedical engineering,king's college london,king's health partners,london,se1 7eh, United Kingdom, university of gothenburg,perinatal center,department of neuroscience and physiology,sahlgrenska academy,box 432,gothenburg,405 30, Sweden, university of gothenburg,perinatal center,department of neuroscience and physiology,sahlgrenska academy,box 432,gothenburg,405 30,sweden,shanghai jiao tong university medical school,luwan branch,department of cardiovascular medicine,ruijin hospital,shanghai, China, university of gothenburg,department of obstetrics and gynecology,sahlgrenska academy,gothenburg,sweden,karolinska institute,department of women's and children's health,karolinska university hospital,stockholm, Sweden, university of gothenburg,perinatal center,department of neuroscience and physiology,sahlgrenska academy,box 432,gothenburg,405 30, Sweden, university of gothenburg,perinatal center,department of neuroscience and physiology,sahlgrenska academy,box 432,gothenburg,405 30, Sweden, university of gothenburg,perinatal center,department of neuroscience and physiology,sahlgrenska academy,box 432,gothenburg,405 30, Sweden, university of gothenburg,perinatal center,department of neuroscience and physiology,sahlgrenska academy,box 432,gothenburg,405 30, Sweden, st. thomas' hospital,department of perinatal imaging and health,division of imaging sciences and biomedical engineering,king's college london,king's health partners,london,se1 7eh, United Kingdom, university of gothenburg,perinatal center,department of neuroscience and physiology,sahlgrenska academy,box 432,gothenburg,405 30,sweden,university of gothenburg,department of obstetrics and gynecology,sahlgrenska academy,gothenburg,sweden,st. thomas' hospital,department of perinatal imaging and health,division of imaging sciences and biomedical engineering,king's college london,king's health partners,london,se1 7eh, United Kingdom, university of gothenburg,perinatal center,department of neuroscience and physiology,sahlgrenska academy,box 432,gothenburg,405 30, Sweden, the university of alabama at birmingham,department of neurosurgery,birmingham,al 35233,united states,the university of alabama at birmingham,department of microbiology,birmingham,al 35233, United States, the third affiliated hospital of zhengzhou university,henan key laboratory of child brain injury,zhengzhou,450052,china,university of gothenburg,department of obstetrics and gynecology,sahlgrenska academy,gothenburg,sweden,university of gothenburg,center for brain repair and rehabilitation,institute of neuroscience and physiology,sahlgrenska academy,box 436,gothenburg,405 30, Sweden, university of gothenburg,perinatal center,department of neuroscience and physiology,sahlgrenska academy,box 432,gothenburg,405 30,sweden,the third affiliated hospital of zhengzhou university,department of pediatrics,zhengzhou, China
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Authors
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